SRK2E | Ser/Thr-protein kinase SnRK2.6

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AS13 2635 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana


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Item No:
AS13 2635

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product information
Background SRK2E (Serine/threonine-protein kinase SRK2E) is an activator of the abscisic acid (ABA) signaling pathway, regulating such ABA responses as: stomato closure, response to drought and plant pathogens.

Synonyms: OST1, Open stomata 1
Immunogen KLH-conjugated inique synthetic peptide derived from Arabidopsis thaliana SRK2E sequence UniProt: Q940H6AT4G33950
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized in PBS pH 7.5
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB), pull-down assay, Immunoprecipitation (IP) (IP)
Related products

AS14 2783 | anti-SRK | Ser/Thr-protein kinase SnRK, rabbit antibodies

AS11 1748 | ABA ELISA quantitation kit

collection of antibodies to proteins involved in signal transduction

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 5 µg (IP), 1 : 1000 (WB) 10 µg (pull-down assay)
Expected | apparent MW

41 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references Wang et al. (2017). Reciprocal Regulation of the TOR Kinase and ABA Receptor Balances Plant Growth and Stress Response. Mol Cell. 2017 Dec 27. pii: S1097-2765(17)30930-9. doi: 10.1016/j.molcel.2017.12.002.

application information

western blot using anti-SRK2E antibodies
Bacterial lysates were separated on 12% SDS-PAGE  and blotted 1h to PVDF using semi-dry or tank transfer. Blots were blocked with 5 % milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 from Agrisera) diluted to 1:50 000 in  for 30 min. at RT with agitation. The blot was washed as above and developed for 3 min with ECL according to the manufacturer's instructions. Exposure time was 30 seconds.

Courtesy of Dr. Agnieszka Ludwików, UAM, Poznań, Poland

pull down using anti-SRK2E antibodies

Protein A agarose beads (40µl) where coated with 10µl (1µg/ul) antibodies and after incubation with amount of extract (10 mg/ml) indicated washed extensively and loaded on gel. In gel kinase assay was performed as described in Fujii, 2007.
Autoradiograph shows immunoprecipitated kinase from plant extracts. 1 beads with BSA 20 µl loaded on gel 2 beads with plant extract (WT) 20 µl loaded on gel 3 beeds with plant extract (mutant X) 20 µl loaded on gel 4 beads with BSA 10 µl loaded on gel 5 beads with plant extract (WT) 10 µl loaded on gel 6 beeds with plant extract (mutant X) 10 µl loaded on gel 7 beads with BSA 5 µl loaded on gel 8 beads with plant extract (WT) 5 µl loaded on gel 9 beeds with plant extract (mutant X) 5 µl loaded on gel

Courtesy of Dr. Szymon Świeżewski, Institute of Biochemistry and Biophysics, Polish Academy of Science, Warsaw, Poland

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