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Epitope tag Antibodies


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AKT1 | Potassium channel AKT1

AS09 516 | Clonality:Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

AKT1 | Potassium channel AKT1 in the group Antibodies for Plant/Algal / Developmental Biology / Ion metabolism at Agrisera AB (Antibodies for research) (AS09 516)

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product information
Background

AKT1 is a highly selective inward-rectifying potassium channel located in cell membrane, that mediates potassium uptake by plant roots in response to low potassium conditions.
Immunogen

KLH-conjugated peptide derived from Arabidopsis thaliana AKT1 Q38998 , At2g26650
Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized
Quantity 200 ĩg
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS09 515 | anti-KC1 | potassium channel KAT3

collection of antibodies to membrane transport system

Plant protein extraction buffer

Secondary antibodies
Additional information For detection images please, refer to the publication belowAntibody detects native and recombinant AKT1.
application information
Recommended dilution 1 : 50 with 125I (WB)
Expected | apparent MW

96.9 kDa
Confirmed reactivity Arabidopsis thaliana
Predicted reactivity

Arabidopsis thaliana
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

In the work of Honsbein et al. 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning. ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems. In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell).
Selected references Safiarian et al. (2015). Lost in traffic? The K+ channel of lily pollen, LilKT1, is detected at the endomembranes inside yeast cells, tobacco leaves and lily pollen. Front. Plant Sci. | doi: 10.3389/fpls.2015.00047.
Honsbein et al. (2009). A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis. The Plant Cell 21:2859-2877.

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