AKT1 | Potassium channel AKT1

Name: AKT1 | Potassium channel AKT1
SKU: AS09 516
Price: 424 EUR
Availability: InStock

AS09 516 | Clonality:Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

AKT1 | Potassium channel AKT1 in the group Antibodies Plant/Algal / Plant Developmental Biology / Ion metabolism at Agrisera AB (Antibodies for research) (AS09 516)

Product Information

Immunogen

KLH-conjugated peptide derived from Arabidopsis thaliana AKT1 Q38998 , At2g26650

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 200 µg

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 50 with 125I (WB)

Expected | apparent MW 96,9 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity

Arabidopsis thaliana

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information For detection images please, refer to the publication belowAntibody detects native and recombinant AKT1

In the work of Honsbein et al, 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning, ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems, In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell)

Background

AKT1 is a highly selective inward-rectifying potassium channel located in cell membrane, that mediates potassium uptake by plant roots in response to low potassium conditions.

Product citations

Selected references Safiarian et al. (2015). Lost in traffic? The K+ channel of lily pollen, LilKT1, is detected at the endomembranes inside yeast cells, tobacco leaves and lily pollen. Front. Plant Sci. | doi: 10.3389/fpls.2015.00047.
Honsbein et al. (2009). A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis. The Plant Cell 21:2859-2877.

immunogen:	KLH-conjugated peptide derived from Arabidopsis thaliana AKT1 Q38998 , At2g26650
Reconstitution:	For reconstitution add 100 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	200 µg
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 50 with 125I (WB)
calculated \| apparent molecular mass [kDa]:	96,9 kDa
Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Arabidopsis thaliana
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	For detection images please, refer to the publication belowAntibody detects native and recombinant AKT1
additional information (application):	In the work of Honsbein et al, 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning, ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems, In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell)
background:	AKT1 is a highly selective inward-rectifying potassium channel located in cell membrane, that mediates potassium uptake by plant roots in response to low potassium conditions.
All references:	Safiarian et al. (2015). Lost in traffic? The K+ channel of lily pollen, LilKT1, is detected at the endomembranes inside yeast cells, tobacco leaves and lily pollen. Front. Plant Sci. \| doi: 10.3389/fpls.2015.00047. Honsbein et al. (2009). A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis. The Plant Cell 21:2859-2877.

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