KC1 | potassium channel KAT3
AS09 515 | Clonality:Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Product Information
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
200 ĩg
Reconstitution
For reconstitution add 100 ĩl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 50 with 125I (WB)
Expected | apparent MW
75,5 | 75 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Arabidopsis thaliana
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Additional information
Additional information
For detection images please, refer to the publication belowAntibody detects native and recombinant KC1
In the work of Honsbein et al, 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning, ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems, In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell)
Background
Background
KC1 is a regulatory K+ channel subunit that assembles with different inward-rectifying K+ channels to affect their activities. The protein is expressed in extremely low amounts, e.g. a few houndred per cell. Alternative names: AKT4, AtKC1, potassium channel TKC
Product citations
Selected references
Honsbein et al. (2009). A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis. The Plant Cell 21:2859-2877.
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