KC1 | potassium channel KAT3

Name: KC1 | potassium channel KAT3
SKU: AS09 515
Price: 424 EUR
Availability: InStock

AS09 515 | Clonality:Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

KC1 | potassium channel KAT3 in the group Antibodies Plant/Algal / Developmental Biology / Ion metabolism at Agrisera AB (Antibodies for research) (AS09 515)

Product Information

Immunogen

KLH-conjugated peptide derived from Arabidopsis thaliana KC1 protein P92960, At4g32650

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 200 µg

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 50 with 125I (WB)

Expected | apparent MW 75,5 | 75 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity

Arabidopsis thaliana

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information For detection images please, refer to the publication belowAntibody detects native and recombinant KC1

In the work of Honsbein et al, 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning, ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems, In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell)

Background

KC1 is a regulatory K+ channel subunit that assembles with different inward-rectifying K+ channels to affect their activities. The protein is expressed in extremely low amounts, e.g. a few houndred per cell. Alternative names: AKT4, AtKC1, potassium channel TKC

Product citations

Selected references Honsbein et al. (2009). A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis. The Plant Cell 21:2859-2877.

background:	KC1 is a regulatory K+ channel subunit that assembles with different inward-rectifying K+ channels to affect their activities. The protein is expressed in extremely low amounts, e.g. a few houndred per cell. Alternative names: AKT4, AtKC1, potassium channel TKC
calculated \| apparent molecular mass [kDa]:	75,5 \| 75 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	KLH-conjugated peptide derived from Arabidopsis thaliana KC1 protein P92960, At4g32650
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Quantity:	200 µg
recommended dilution:	1 : 50 with 125I (WB)
Reconstitution:	For reconstitution add 100 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Arabidopsis thaliana
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	For detection images please, refer to the publication belowAntibody detects native and recombinant KC1
additional information (application):	In the work of Honsbein et al, 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning, ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems, In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell)
All references:	Honsbein et al. (2009). A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis. The Plant Cell 21:2859-2877.

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