HSP70B | Stromal alfa-HSP70 (algal)

AS06 175 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Ch. reinhardtii, D. subspicatus, P. patens

Product Information

Immunogen

mature HSP70B protein UniProt: A8HYV3, expressed with N- and C-terminal hexahistidine tags in E. coli, purified with Ni-NTA

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunoprecipitation (IP), Western blot (WB)

Recommended dilution 1 : 10 000 (WB)

Expected | apparent MW 71,9 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii, Desmodesmus subspicatus, Physcomitrium patens

Predicted reactivity Dunaliella salina, Cyanobacteria
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Reactant: Chlamydomonas reinhardtii (Green Alga)

Application: Western Blotting

Pudmed ID: 23118617

Journal: PLoS Biol

Figure Number: 5A

Published Date: 2012-11-03

First Author: Teng, Y. S., Chan, P. T., et al.

Impact Factor: 7.279

Open Publication

Protein import into Chlamydomonas chloroplasts is not differentially regulated.[35S]Met-labeled precursors of Cr-prRBCS, Cr-prL11, prOE23, prTic40, and prL11 were imported into chloroplasts isolated from synchronized cultures of Chlamydomonas 1, 6, and 11 h after the start of the third light cycle and used for import experiments. Samples were analyzed by SDS-PAGE and autoradiography. An equal number of chloroplasts were loaded in each lane of the same gel. The amount of Chlamydomonas chloroplast Hsp70B was analyzed by immunoblotting and used for normalization of quantifications in the bar graph shown below each gel. The amount of mature protein imported at hour 1 was set as 100%. Data shown are mean ± SD, n?=?3. m, mature form; p, precursor form; TR, in vitro–translated precursor proteins before import.

Additional information

Background

HSP70B is a nuclear-encoded, chloroplast-targeted chaperone of the HSP70 family. It is the major HSP70 in the stroma of Chlamydomonas reinhardtii chloroplasts. It interacts with HSP90C, CGE1, CDJ2, and VIPP1.

Product citations

Selected references Cvetkovska et al. (2022) A constitutive stress response is a result of low temperature growth in the Antarctic green alga Chlamydomonas sp. UWO241. Plant, Cell & Environment, 45, 156– 177. https://doi.org/10.1111/pce.14203
Gonzaga Heredia-Martinez et al. (2018). Chloroplast damage induced by the inhibition of fatty acid synthesis triggers autophagy in Chlamydomonas. Plant Physiol, Sept. 2018.
Diaz-Troya et al. (2011). Inhibition of protein synthesis by TOR inactivation revealed a conserved regulatory mechanism of the BiP chaperone in Chlamydomonas. Plant Physiol, in press.

More images:	Reactant: Chlamydomonas reinhardtii (Green Alga) Application: Western Blotting Pudmed ID: 23118617 Journal: PLoS Biol Figure Number: 5A Published Date: 2012-11-03 First Author: Teng, Y. S., Chan, P. T., et al. Impact Factor: 7.279 Open Publication Protein import into Chlamydomonas chloroplasts is not differentially regulated.[35S]Met-labeled precursors of Cr-prRBCS, Cr-prL11, prOE23, prTic40, and prL11 were imported into chloroplasts isolated from synchronized cultures of Chlamydomonas 1, 6, and 11 h after the start of the third light cycle and used for import experiments. Samples were analyzed by SDS-PAGE and autoradiography. An equal number of chloroplasts were loaded in each lane of the same gel. The amount of Chlamydomonas chloroplast Hsp70B was analyzed by immunoblotting and used for normalization of quantifications in the bar graph shown below each gel. The amount of mature protein imported at hour 1 was set as 100%. Data shown are mean ± SD, n?=?3. m, mature form; p, precursor form; TR, in vitro–translated precursor proteins before import.
calculated \| apparent molecular mass [kDa]:	71,9 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	mature HSP70B protein UniProt: A8HYV3, expressed with N- and C-terminal hexahistidine tags in E. coli, purified with Ni-NTA
Purity:	Serum
Quantity:	100 µl
recommended dilution:	1 : 10 000 (WB)
Reconstitution:	For reconstitution add 100 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Immunoprecipitation (IP), Western blot (WB)
All references:	Cvetkovska et al. (2022) A constitutive stress response is a result of low temperature growth in the Antarctic green alga Chlamydomonas sp. UWO241. Plant, Cell & Environment, 45, 156– 177. https://doi.org/10.1111/pce.14203 Gonzaga Heredia-Martinez et al. (2018). Chloroplast damage induced by the inhibition of fatty acid synthesis triggers autophagy in Chlamydomonas. Plant Physiol, Sept. 2018. Diaz-Troya et al. (2011). Inhibition of protein synthesis by TOR inactivation revealed a conserved regulatory mechanism of the BiP chaperone in Chlamydomonas. Plant Physiol, in press.
background:	HSP70B is a nuclear-encoded, chloroplast-targeted chaperone of the HSP70 family. It is the major HSP70 in the stroma of Chlamydomonas reinhardtii chloroplasts. It interacts with HSP90C, CGE1, CDJ2, and VIPP1.
Confirmed reactivity:	Chlamydomonas reinhardtii, Desmodesmus subspicatus, Physcomitrium patens
predicted reactivity:	Dunaliella salina, Cyanobacteria Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known

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