Anti-AMY | Alpha-amylase

Product no: AS10 712

AS10 712  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Oryza sativa

Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from known Oryza sativa P17654

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 100 µl
    Reconstitution: For reconstitution add 100 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 5000 (WB)
    Expected | apparent MW:

    43-48 kDa (depending upon the isoform)

  • Reactivity
  • Confirmed reactivity: Oryza sativa, Panicum virgatum
    Predicted reactivity:

    Cereals, Hordeum vulgare, Kalanchoe laxiflora

    Species of your interest not listed? Contact us
    Not reactive in:

    Pinus strobus

  • Application Examples
  • Application examples

    alpha-Ramy detection in rice using western blot

    25 µg of total protein from Oryza sativa seedlings (from 1 day to 5 days of imbibition on filter paper at 28°C) extracted with an SDS Extraction Buffer (60mM Tris-HCl pH 8.0, 2% SDS, 1,5% Sucrose) were separated on XT CRITERION 10%Bis-Tris (BioRad) SDS-PAGE and blotted 1h to PVDF. Blot was blocked immediately in milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a diluition of 1: 5,000 in milk in TBS-T for 3h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly once, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG HRP conjugated, AS09 602) diluited 1:20 000 in milk in TBS-T for 50 min at RT and then washed as above and developed for 3 min with chemiluminescent detection. Images of the blot were obtained using BioSpectrum AC Imaging System (UVP). Exposure time was 30 min. The arrow indicates alpha-amylase (between 47kDa and 50KDa as expected).

    Courtesy Valeria Banti and prof. Pierodomenico Perata, PlantLab, Scuola Superiore Sant'Anna

  • Additional Information
  • Additional information: Antibody will detect all alpha amylase isoforms from rice, barley and other cereals
  • Background
  • Background:

    α-Amylases are hydrolytic enzymes responsible for the mobilization of the starch into metabolizable sugars. This process provides the energy for the growth of roots and shoots and is crucial during germination of cereal seeds.These enzymes are coded by a multigene family and even thought other amylolytic enzyme participate in the process of starch breakdown, the contribution of α-amylase is the prerequisite for the initiation of this process.

  • Product Citations
  • Selected references: Ye et al. (2018). Natural variation in the promoter of rice calcineurin B-like protein10 (OsCBL10) affects flooding tolerance during seed germination among rice subspecies. Plant J. 2018 May;94(4):612-625. doi: 10.1111/tpj.13881.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (0)

    This product doesn't have any reviews.


AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

213 €
Add to cart
img missing

AS10 716  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Oryza sativa

448 €
Add to cart