SOD1 aa 80-96 | superoxide dismutase 1, soluble

AS13 2645  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: Human

SOD1 aa 80-96 | superoxide dismutase 1, soluble in the group Human/Animal Antibodies / Human Proteins / Oxidative stress at Agrisera AB (Antibodies for research) (AS13 2645)


332 €

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product information

SOD1 [Cu-Zn] (EC= is a cytoplasm localized oxidoreductase which destroys radicals normally produced within the cells and toxic to biological systems. Alternative names: SOD, soluble, indophenoloxidase A, Cu/Zn superoxide dismutase, superoxide dismutase, cytosolic.


KLH-conjugated synthetic peptide derived from human SOD1 sequence, amino acids 80-96 P00441

Peptide used to elicit this antibody is not conserved in SOD2, 3 and 4.

Host Mouse
Clonality Monoclonal
Clone IgG1, (clone number 210.29)
Purity Affinity purified
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Related products

AS09 538 | SOD1 aa 80-96 | superoxide dismutase 1, soluble, rabbit polyclonal antibody

AS13 2643 | SOD1 aa 24-39 | superoxide dismutase 1, soluble, mouse monoclonal antibody

AS13 2644 | SOD1 aa 58-72 | superoxide dismutase 1, soluble, mouse monoclonal antibody

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000-1 : 10 000 (ELISA), 1 : 1000 (WB)
Expected | apparent MW

15.9 kDa

Confirmed reactivity Human
Predicted reactivity Bovine, Chimpanzee, Dog, Goat, Guinea Pig, Mouse, Pig, Rabbit, Rat, Schizosaccharomyces pombe, Sheep
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references

to be added when available, antibody released in March 2013.

application example

western blot detection of SOD1 80-96 using a mouse monoclonal antibody

100,10 and 1 ng of recombinant human SOD1 were separated by 4-20 % SDS-PAGE and transferred electrophoretically (25V, 10 min) onto PVDF membrane. Non-specific binding sites were blocked by incubating membrane with 5 % dry milk in PBS, 0.1 % Tween 20 for 1h at room temperature (RT) with agitation. The membrane was thereafter incubated with the primary antibody SOD1 aa 58-72 at a dilution of 1: 1 000 for 3h at RT with agitation. The antibody solution was decanted and the membrane was rinsed 3 times for 5 min in PBS-T (0.05 %) at RT with agitation. The membrane was then incubated with the secondary antibody (Rabbit Anti-Mouse IgG – HRP conjugated (DAKO) at a 1:1 000 dilution) for 1h at RT with agitation. The membrane was washed as above and developed for 5 min with Amersham ECL prime western blotting detection reagent according to the manufacturer’s instructions (GE Healthcare). Exposure time was 15 s.

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