SOD1 aa 58-72 | Superoxide dismutase 1, soluble (clone number 15,13)

Name: SOD1 aa 58-72 | Superoxide dismutase 1, soluble (clone number 15,13)
SKU: AS13 2644
Price: 369 EUR
Availability: InStock

AS13 2644 | Clonality: monoclonal | Host: Mouse | Reactivity: Human

SOD1 aa 58-72 | Superoxide dismutase 1, soluble (clone number 15,13) in the group Antibodies Human Cell Biology / Human Oxidative Stress at Agrisera AB (Antibodies for research) (AS13 2644)

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from human SOD1 sequence, amino acids 58-72 P00441

Peptide used to elicit this antibody is not conserved in SOD2, 3 and 4.

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG1

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1 : 1000-1 : 10 000 (ELISA), 1 : 1000 (WB)

Expected | apparent MW 15,9 kDa

Reactivity

Confirmed reactivity Human

Predicted reactivity Bovine, Chimpanzee, Dog, Goat, Guinea Pig, Mouse, Pig, Rabbit, Rat, Schizosaccharomyces pombe, Sheep

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

application example

100,10 and 1 ng of recombinant human SOD1 were separated by 4-20 % SDS-PAGE and transferred electrophoretically (25V, 10 min) onto PVDF membrane. Non-specific binding sites were blocked by incubating membrane with 5 % dry milk in PBS, 0.1 % Tween 20 for 1h at room temperature (RT) with agitation. The membrane was thereafter incubated with the primary antibody SOD1 aa 58-72 at a dilution of 1: 1 000 for 3h at RT with agitation. The antibody solution was decanted and the membrane was rinsed 3 times for 5 min in PBS-T (0.05 %) at RT with agitation. The membrane was then incubated with the secondary antibody (Rabbit Anti-Mouse IgG – HRP conjugated (DAKO) at a 1:1 000 dilution) for 1h at RT with agitation. The membrane was washed as above and developed for 5 min with Amersham ECL prime western blotting detection reagent according to the manufacturer’s instructions (GE Healthcare). Exposure time was 15 s.

Additional information

Background

SOD1 [Cu-Zn] (EC=1.15.1.1) is a cytoplasm localized oxidoreductase which destroys radicals normally produced within the cells and toxic to biological systems. Alternative names: SOD, soluble, indophenoloxidase A, Cu/Zn superoxide dismutase, superoxide dismutase, cytosolic.

Product citations

immunogen:	KLH-conjugated synthetic peptide derived from human SOD1 sequence, amino acids 58-72 P00441 Peptide used to elicit this antibody is not conserved in SOD2, 3 and 4.
Reconstitution:	For reconstitution add 50 µl of sterile water
Sub class:	IgG1
Host:	Mouse
Clonality:	Monoclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	50 µg
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Western blot (WB)
recommended dilution:	1 : 1000-1 : 10 000 (ELISA), 1 : 1000 (WB)
calculated \| apparent molecular mass [kDa]:	15,9 kDa
Confirmed reactivity:	Human
predicted reactivity:	Bovine, Chimpanzee, Dog, Goat, Guinea Pig, Mouse, Pig, Rabbit, Rat, Schizosaccharomyces pombe, Sheep
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	application example 100,10 and 1 ng of recombinant human SOD1 were separated by 4-20 % SDS-PAGE and transferred electrophoretically (25V, 10 min) onto PVDF membrane. Non-specific binding sites were blocked by incubating membrane with 5 % dry milk in PBS, 0.1 % Tween 20 for 1h at room temperature (RT) with agitation. The membrane was thereafter incubated with the primary antibody SOD1 aa 58-72 at a dilution of 1: 1 000 for 3h at RT with agitation. The antibody solution was decanted and the membrane was rinsed 3 times for 5 min in PBS-T (0.05 %) at RT with agitation. The membrane was then incubated with the secondary antibody (Rabbit Anti-Mouse IgG – HRP conjugated (DAKO) at a 1:1 000 dilution) for 1h at RT with agitation. The membrane was washed as above and developed for 5 min with Amersham ECL prime western blotting detection reagent according to the manufacturer’s instructions (GE Healthcare). Exposure time was 15 s.
background:	SOD1 [Cu-Zn] (EC=1.15.1.1) is a cytoplasm localized oxidoreductase which destroys radicals normally produced within the cells and toxic to biological systems. Alternative names: SOD, soluble, indophenoloxidase A, Cu/Zn superoxide dismutase, superoxide dismutase, cytosolic.