SOD1 aa 58-72 | Superoxide dismutase 1, soluble (clone number 15,13)

Product no: AS13 2644

AS13 2644  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: Human

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Delivery:  3-6 business days
  • Product Info
  • Sub class: IgG1
    Immunogen:

    KLH-conjugated synthetic peptide derived from human SOD1 sequence, amino acids 58-72 P00441

    Peptide used to elicit this antibody is not conserved in SOD2, 3 and 4.

    Host: Mouse
    Clonality: Monoclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Western blot (WB)
    Recommended dilution: 1 : 1000-1 : 10 000 (ELISA), 1 : 1000 (WB)
    Expected | apparent MW: 15,9 kDa
  • Reactivity
  • Confirmed reactivity: Human
    Predicted reactivity: Bovine, Chimpanzee, Dog, Goat, Guinea Pig, Mouse, Pig, Rabbit, Rat, Schizosaccharomyces pombe, Sheep
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example

    western blot detection of SOD1 58-72 using a mouse monoclonal antibody

    100,10 and 1 ng of recombinant human SOD1 were separated by 4-20 % SDS-PAGE and transferred electrophoretically (25V, 10 min) onto PVDF membrane. Non-specific binding sites were blocked by incubating membrane with 5 % dry milk in PBS, 0.1 % Tween 20 for 1h at room temperature (RT) with agitation. The membrane was thereafter incubated with the primary antibody SOD1 aa 58-72 at a dilution of 1: 1 000 for 3h at RT with agitation. The antibody solution was decanted and the membrane was rinsed 3 times for 5 min in PBS-T (0.05 %) at RT with agitation. The membrane was then incubated with the secondary antibody (Rabbit Anti-Mouse IgG – HRP conjugated (DAKO) at a 1:1 000 dilution) for 1h at RT with agitation. The membrane was washed as above and developed for 5 min with Amersham ECL prime western blotting detection reagent according to the manufacturer’s instructions (GE Healthcare). Exposure time was 15 s.

     

  • Background
  • Background:

    SOD1 [Cu-Zn] (EC=1.15.1.1) is a cytoplasm localized oxidoreductase which destroys radicals normally produced within the cells and toxic to biological systems. Alternative names: SOD, soluble, indophenoloxidase A, Cu/Zn superoxide dismutase, superoxide dismutase, cytosolic.

  • Product Citations
  • Selected references: 38221900
  • Protocols
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