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SOD1 aa 24-39 | superoxide dismutase 1, soluble (clone number 6D4.G9.C3.2)

AS13 2643  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: Human

SOD1 aa 24-39 | superoxide dismutase 1, soluble (clone number 6D4.G9.C3.2) in the group Antibodies for Human/Animal  / Human Proteins / Oxidative stress at Agrisera AB (Antibodies for research) (AS13 2643)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from human SOD1 sequence P00441

Peptide used to elicit this antibody is not conserved in SOD2, 3 and 4.

Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Affinity purified in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 1000-1 : 10 000 (ELISA), 1 : 1000 (WB)
Expected | apparent MW

15.9 kDa

Reactivity

Confirmed reactivity Human
Predicted reactivity Chimpanzee, Schizosaccharomyces pombe
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

western blot detection of SOD1 aa24-39 using a monoclonal antibody

 

100,10 and 1 µg of recombinant human SOD1 were separated by 4-20 % SDS-PAGE and transferred electrophoretically (25V, 10 min) onto PVDF membrane. Non-specific binding sites were blocked by incubating membrane with 5 % dry milk in PBS, 0.1 % Tween 20 for 1h at room temperature (RT) with agitation. The membrane was thereafter incubated with the primary antibody SOD1 aa 24-39 at a dilution of 1: 1 000 for 3h at RT with agitation. The antibody solution was decanted and the membrane was rinsed 3 times for 5 min in PBS-T (0.05 %) at RT with agitation. The membrane was then incubated with the secondary antibody (Rabbit Anti-Mouse IgG – HRP conjugated (DAKO) at a 1:1 000 dilution) for 1h at RT with agitation. The membrane was washed as above and developed for 5 min with Amersham ECL prime western blotting detection reagent according to the manufacturer’s instructions (GE Healthcare). Exposure time was 1 s.

Additional information

Related products

Related products

AS13 2644 | Anti-SOD1 aa 58-72 | superoxide dismutase 1, soluble, rabbit antibodies

AS13 2645 | Anti-SOD1 aa 80-96 | superoxide dismutase 1, soluble, rabbit antibodies

polyclonal antibodies to human SOD1

Secondary antibodies

Background

Background

SOD1 [Cu-Zn] (EC=1.15.1.1) is a cytoplasm localized oxidoreductase which destroys radicals normally produced within the cells and toxic to biological systems.

Alternative names: SOD, soluble, indophenoloxidase A, Cu/Zn superoxide dismutase, superoxide dismutase, cytosolic.

Product citations

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