SOD1 aa 24-39 | superoxide dismutase 1, soluble (clone number 6D4,G9,C3,2)

Product no: AS13 2643

AS13 2643  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: Human

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from human SOD1 sequence P00441

    Peptide used to elicit this antibody is not conserved in SOD2, 3 and 4.

    Sub class: IgG1
    Host: Mouse
    Clonality: Monoclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Western blot (WB)
    Recommended dilution: 1 : 1000-1 : 10 000 (ELISA), 1 : 1000 (WB)
    Expected | apparent MW: 15,9 kDa
  • Reactivity
  • Confirmed reactivity: Human
    Predicted reactivity: Chimpanzee, Schizosaccharomyces pombe
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example

    western blot detection of SOD1 aa24-39 using a monoclonal antibody

     

    100,10 and 1 µg of recombinant human SOD1 were separated by 4-20 % SDS-PAGE and transferred electrophoretically (25V, 10 min) onto PVDF membrane. Non-specific binding sites were blocked by incubating membrane with 5 % dry milk in PBS, 0.1 % Tween 20 for 1h at room temperature (RT) with agitation. The membrane was thereafter incubated with the primary antibody SOD1 aa 24-39 at a dilution of 1: 1 000 for 3h at RT with agitation. The antibody solution was decanted and the membrane was rinsed 3 times for 5 min in PBS-T (0.05 %) at RT with agitation. The membrane was then incubated with the secondary antibody (Rabbit Anti-Mouse IgG – HRP conjugated (DAKO) at a 1:1 000 dilution) for 1h at RT with agitation. The membrane was washed as above and developed for 5 min with Amersham ECL prime western blotting detection reagent according to the manufacturer’s instructions (GE Healthcare). Exposure time was 1 s.

  • Background
  • Background:

    SOD1 [Cu-Zn] (EC=1.15.1.1) is a cytoplasm localized oxidoreductase which destroys radicals normally produced within the cells and toxic to biological systems.

    Alternative names: SOD, soluble, indophenoloxidase A, Cu/Zn superoxide dismutase, superoxide dismutase, cytosolic.

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