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Anti-MSH2 | DNA mismatch repair protein MSH2
AS24 5012 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Triticum sp.
- Product Info
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Immunogen: KLH-conjugated peptide derived from MSH2 protein sequence of Arabidopsis thaliana, UniProt: O24617 GeneID: AT3G18524 Host: Rabbit Clonality: Polyclonal Purity: Antigen affinity purified serum, in PBS pH 7.4 Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution, add 50 µl, of sterile or deionized water. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications: Immunofluorescence (IF) Recommended dilution: 1 : 200 (IF) Expected | apparent MW: 105.5 kDa - Reactivity
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Confirmed reactivity: Triticum aestivum Predicted reactivity: Arabidopsis thaliana, Arachis hypogaea, Capsicum annuum, Citrus sp., Cucumis sativus, Gossypium sp., Malus domestica, Medicago truncatula, Nicotiana tabacum, Pisum sativum, Populus sp., Solanum lycopersicum, Spinacia oleracea, Solanum tuberosum, Theobroma cacao
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Applied procedure:
Type of material: Meiocytes of Triticum aestivum
Fixation: 3:1 (ethanol : acetic acid)
Hydrophilization: no
Cell wall digestion: no
Membrane permeabilization: PBST (PBS1X, 0.1% Triton X-100)
Antigen retrieval: yes
Blocking buffer: 1% BSA in PBST
Washing buffer: PBST
Primary antibody dilution and incubation time: 1:200, 40 H
Secondary antibody:1:400, 30 min, Thermo Fischer Scientific
Co-staining of the nucleus (DAPI): yes
Cell wall and nucleus staining: noCourtesy of Floriane Chéron, INRAE UMR1095, France
- Background
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Background: MSH2 (DNA mismatch repair protein MSH2) is a component of the post-replicative DNA mismatch repair system (MMR). Forms three different heterodimers: MutS alpha (MSH2-MSH6 heterodimer), MutS beta (MSH2-MSH3 heterodimer) and MutS gamma (MSH2-MSH7 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS15 3018 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Acetylated Lysine
AS24 5042| Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
This set includes three discounted, well published, compartment marker antibodies of your choice. Benefits of using this antibody set
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