Anti-SYP121 | Syntaxin 121

Product no: AS22 4827

AS22 4827 | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana

Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana SYP121 protein sequence, UniProt: Q9ZSD4-1, TAIR: AT3G11820
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Antigen affinity purified serum, in PBS pH 7.4
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution add 50 µl, of sterile or deionized water.
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 37.9  kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Brassica napus, Brassica oleracea, Capsicum annum, Euphorbia peplus, Glycine max, Gossypium sp., Hirschfeldia incana, Jatropha curcas, Malus domestica, Manihot esculenta, Medicago truncatula, Nicotiana tabacum, Pisum sativum, Raphanus sativus, Solanum tuberosum, Spinacia oleracea, Theobroma cacao, Vitis vinifera

    Species of your interest not listed? Contact us
    Not reactive in: Dunaliella salina, Triticum aestivum
  • Application Examples
  • Western blot using anti-SYP121 antibodies

    Marker: Precision Plus Dual Color Standard (Biorad)
    1-20 µg of Arabidopsis thaliana total protein extract
    2-20 µg of Arabidopsis thaliana cytosolic fraction
    3-20 µg of Arabidopsis thaliana microsomal fraction
    4-20 µg of Arabidopsis thaliana plasma membrane fraction
    5-20 µg of Arabidopsis thaliana other membranes fraction

    Arabidopsis thaliana plants were grown for 13 days at 22°C with a time regime of 16hday/8h night. Plants were grown on 1/2MS agar plates, pH=5.8. Whole plants were used for the experiment.

    Samples were extracted with 50 mM HEPES pH 7,5, 400 mM sucrose, 100 mM KCl, 100 mM MgCl2, Complete Protease Inhibitor Cocktail; 3-8 resuspend with 5 mM phosphate buffer pH 7,8 and denaturated with Laemmli sample buffer (Biorad) with XT reducing agent (Biorad) at 65 °C (1-5) or 70 °C (6-8) for 5 min. Samples were separated on 4-20% Mini-PROTEAN TGX stain free precast gel and blotted 7 min to PVDF (pore size of 0,2 µm) using Turbo Blot. Blot was blocked with 5% milk in TBS-T for 1h/RT with agitation. Blot was incubated with antiSYP121 (AS 22 4827) at a dilution 1:1000 ON/4°C in TBS-T. The antibody solution was decanted and the blot was washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Goat anti-Rabbit IgG HRP conjugated (Agrisera AS09602-trial) diluted to 1:25000 in 5% milk in TBS-T for 1h/RT with agitation. The blot was washed as above and developed for 5 min with Agrisera ECLBright. Exposure time was 2 seconds.

    Courtesy of Dr. Roman Hudeček, Integrative Structural Biology, IEB CAS CZ, Czech Republic

  • Additional Information
  • Additional information (application): This antibody is also recognizing recombinant SYP121-GFP.
  • Background
  • Background: SYP121 (Syntaxin 121) is a syntaxin localized at the plasma membrane. SYR1/PEN1 is a member of the SNARE superfamily and functions in positioning anchoring of the KAT1 K+ channel protein at the plasma membrane.

    Alternative protein name: Protein SYNTAXIN OF PLANTS 121, Syntaxin-related protein At-Syr1
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (0)

    This product doesn't have any reviews.


AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

213 €
Add to cart

AS07 260 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for di- and monocots, conifers, ferns, mosses, green algae | Cellular [compartment marker] for plasma membrane

Benefits of using this antibody

319 €
Add to cart
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items

AS13 2708  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

319 €
Add to cart
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items