ANN-1 | Annexin-1
AS18 4166 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Product Information
Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana Annexin-1, UniProt Q9SYT0, TAIR AT1G35720
Host
Rabbit
Clonality
Polyclonal
Purity
Affinity purified serum, in PBS pH 7.4
Format
Lyophilized
Quantity
50 µg
Reconstitution
For reconstitution add 50 µl, of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
36 kDa (before processing)
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
Application example
Recombinant Ann1 (1), Ann4 OE (2). Leaves of Arabidopsis thaliana were ground in liquid nitrogen and total protein was extracted using extraction buffer: Tris pH-8.0 (1M) 5%, EDTA pH-8.0 (0.5 M) 0.2%, β-Mercaptoethanol 0.05%, PMSF (1%) 0.05%, Triton X-100 1%, in distilled water. For each sample, 50 µg of extracted protein was taken, diluted with distilled water and added with 4xLaemli Sample buffer. The samples were denatured at 95℃ for 5 min and were separated on 12 % SDS-PAGE. The samples were blotted for 2 h to PVDF membrane using tank/wet transfer at 4℃ with constant stirring. The blots were blocked with blocking solution (5% dried milk in 1xTBS) for overnight at 4℃ with agitation. Blots were incubated in the primary antibody at a dilution of 1:1000 for 1h at RT with agitation in 5% dried milk in 1xTBS. The antibody solution was decanted and the blots were rinsed briefly, then washed once for 15 min and 3 times for 5 min in 1xTBS at RT with agitation. Blots were incubated in secondary antibody (Goat anti-rabbit IgG (HRP conjugated)) diluted to 1:50 000 in 5% dried milk in 1xTBS for 1h at RT with agitation. The blots were washed as mentioned above and developed for 5 min with chemiluminescent detection reagent in dark room. The exposure time was 10 min.
Courtesy Dr. dr Umesh Tanwar, Departament of Plant Biochemistry Institute of Biochemistry and Biophysics PAS, Poland
Recombinant Ann1 (1), Ann4 OE (2). Leaves of Arabidopsis thaliana were ground in liquid nitrogen and total protein was extracted using extraction buffer: Tris pH-8.0 (1M) 5%, EDTA pH-8.0 (0.5 M) 0.2%, β-Mercaptoethanol 0.05%, PMSF (1%) 0.05%, Triton X-100 1%, in distilled water. For each sample, 50 µg of extracted protein was taken, diluted with distilled water and added with 4xLaemli Sample buffer. The samples were denatured at 95℃ for 5 min and were separated on 12 % SDS-PAGE. The samples were blotted for 2 h to PVDF membrane using tank/wet transfer at 4℃ with constant stirring. The blots were blocked with blocking solution (5% dried milk in 1xTBS) for overnight at 4℃ with agitation. Blots were incubated in the primary antibody at a dilution of 1:1000 for 1h at RT with agitation in 5% dried milk in 1xTBS. The antibody solution was decanted and the blots were rinsed briefly, then washed once for 15 min and 3 times for 5 min in 1xTBS at RT with agitation. Blots were incubated in secondary antibody (Goat anti-rabbit IgG (HRP conjugated)) diluted to 1:50 000 in 5% dried milk in 1xTBS for 1h at RT with agitation. The blots were washed as mentioned above and developed for 5 min with chemiluminescent detection reagent in dark room. The exposure time was 10 min.
Courtesy Dr. dr Umesh Tanwar, Departament of Plant Biochemistry Institute of Biochemistry and Biophysics PAS, Poland
Additional information
Antibody is detecting recombinant Annexin-1 of Arabidopsis thaliana. Its reactivity on endogenous form remains to be confirmed.
Background
Background
ANN-1 | Annexin-1 displays peroxidase activity and may counteract oxidative stress.
Alternative names: ANNAT1, ANX23-ATH, ATOXY5, OXY5, Annexin D1, AnnAt1.
Alternative names: ANNAT1, ANX23-ATH, ATOXY5, OXY5, Annexin D1, AnnAt1.
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