ATG7 | Ubiquitin-like modifier-activating enzyme atg7

AS15 3061 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

ATG7 | Ubiquitin-like modifier-activating enzyme atg7

Product Information

Immunogen Recombinant Arabidopsis thaliana ATG7 UniProt Q94CD5, TAIR AT5G45900

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution

1: 500 - 1: 5000 (WB)

Expected | apparent MW 76,5 | 80 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Actinidia chinensis var. chinensis, Cajanus cajan, Capsicum annuum, Corchorus olitorius, Cucumis melo, Glycine max, Glycine soja, Gossypium arboreum, Juglans regia, Malus domestica, Nelumbo nucifera, Nicotiana tabacum, Nicotiana benthamiana, Nicotiana sylvestris, Noccaea caerulescens, Prunus yedoensis var. nudiflora, Solanum chacoense, Solanum lycopersicum, Vigna radiata
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

Western blot using anti-ATG7 antibodies

50 µg of the soluble proteins (Sol) were extracted from Arabidopsis thaliana leaves with extract buffer without detergent (50mM Tris-HCl pH7.5, 150mM NaCl, 1mM EDTA). The proteins were denatured with sample buffer and boiling at 95°C for 5 min and 50 ug of proteins were separated on 10% SDS-PAGE and blotted for 1h to PVDF membrane using semi-dry transfer. The blot was blocked with 0.5 % milk for 1h/RT and washed in TBS-T for 5 minute twice. The blot was incubated in the primary antibody at a dilution of 1:5 000 in Can Get Signal solution for ON/4°C. The antibody solution was decanted and the blot was rinsed briefly three times, then washed once for 15 min in TBS-T at RT with agitation. The blot was incubated in Agrisera matching secondary antibody (anti-rabit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:25 000 in TBS-T for 1h/RT with agitation. The blot was washed as above and developed for 3 min with Agrisera ECL SuperBright.

Exposure Courtesy Dr Shino Goto-Yamada, Malopolska Centre of Biotechnology (MCB) Jagiellonian University, Krakow, Poland

Additional information

CanGetSignal (Toyobo) is recommended for antibody incubation

Background

Product citations

calculated \| apparent molecular mass [kDa]:	76,5 \| 80 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	Recombinant Arabidopsis thaliana ATG7 UniProt Q94CD5, TAIR AT5G45900
Purity:	Serum
Quantity:	50 µl
recommended dilution:	1: 500 - 1: 5000 (WB)
Reconstitution:	For reconstitution add 50 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Actinidia chinensis var. chinensis, Cajanus cajan, Capsicum annuum, Corchorus olitorius, Cucumis melo, Glycine max, Glycine soja, Gossypium arboreum, Juglans regia, Malus domestica, Nelumbo nucifera, Nicotiana tabacum, Nicotiana benthamiana, Nicotiana sylvestris, Noccaea caerulescens, Prunus yedoensis var. nudiflora, Solanum chacoense, Solanum lycopersicum, Vigna radiata Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	Application example 50 µg of the soluble proteins (Sol) were extracted from Arabidopsis thaliana leaves with extract buffer without detergent (50mM Tris-HCl pH7.5, 150mM NaCl, 1mM EDTA). The proteins were denatured with sample buffer and boiling at 95°C for 5 min and 50 ug of proteins were separated on 10% SDS-PAGE and blotted for 1h to PVDF membrane using semi-dry transfer. The blot was blocked with 0.5 % milk for 1h/RT and washed in TBS-T for 5 minute twice. The blot was incubated in the primary antibody at a dilution of 1:5 000 in Can Get Signal solution for ON/4°C. The antibody solution was decanted and the blot was rinsed briefly three times, then washed once for 15 min in TBS-T at RT with agitation. The blot was incubated in Agrisera matching secondary antibody (anti-rabit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:25 000 in TBS-T for 1h/RT with agitation. The blot was washed as above and developed for 3 min with Agrisera ECL SuperBright. Exposure Courtesy Dr Shino Goto-Yamada, Malopolska Centre of Biotechnology (MCB) Jagiellonian University, Krakow, Poland
additional information (application):	CanGetSignal (Toyobo) is recommended for antibody incubation