Name:
Phone:
E-mail:
Message

ATG7 | Ubiquitin-like modifier-activating enzyme atg7

AS15 3061 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

ATG7 | Ubiquitin-like modifier-activating enzyme atg7 in the group Antibodies for Plant/Algal  / Developmental Biology / Senescence/Cell death at Agrisera AB (Antibodies for research) (AS15 3061)

DATA SHEET IN PDF

Qty: 
278
Buy 2 items of this product for 194.00 €/items
Buy 3 items of this product for 178.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Datasheet Product citations Protocols Add review

Product Information

Immunogen Recombinant Arabidopsis thaliana ATG7 UniProt Q94CD5, TAIR AT5G45900
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution

For reconstitution add 50 µl of sterile water.

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Recommended dilution

1: 500 - 1: 5000 (WB)

Expected | apparent MW

76.5 | 80 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Actinidia chinensis var. chinensis, Cajanus cajan, Capsicum annuum, Corchorus olitorius, Cucumis melo, Glycine max, Glycine soja, Gossypium arboreum, Juglans regia, Malus domestica, Nelumbo nucifera, Nicotiana tabacum, Nicotiana benthamiana, Nicotiana sylvestris, Noccaea caerulescens, Prunus yedoensis var. nudiflora, Solanum chacoense, Vigna radiata
Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Application examples

Application examples Application example


Western blot using anti-ATG7 antibodies

50 µg of the soluble proteins (Sol) were extracted from Arabidopsis thaliana leaves with extract buffer without detergent (50mM Tris-HCl pH7.5, 150mM NaCl, 1mM EDTA). The proteins were denatured with sample buffer and boiling at 95°C for 5 min and 50 ug of proteins were separated on 10% SDS-PAGE and blotted for 1h to PVDF membrane using semi-dry transfer. The blot was blocked with 0.5 % milk for 1h/RT and washed in TBS-T for 5 minute twice. The blot was incubated in the primary antibody at a dilution of  1:5 000 in Can Get Signal solution for ON/4°C. The antibody solution was decanted and the blot was rinsed briefly three times, then washed once for 15 min in TBS-T at RT with agitation. The blot was incubated in Agrisera matching secondary antibody (anti-rabit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:25 000 in TBS-T for 1h/RT with agitation. The blot was washed as above and developed for 3 min with Agrisera ECL SuperBright.

Exposure Courtesy Dr Shino Goto-Yamada, Malopolska Centre of Biotechnology (MCB) Jagiellonian University, Krakow, Poland

Additional information

CanGetSignal (Toyobo) is recommended for antibody incubation.

Related products

Related products

AS14 2811 | Anti-ATG8A | Autophagy-related protein 8a, rabbit antibodies
AS14 2769 | Anti-ATG8 | Autophagy-related protein, rabbit antibodies

Plant and algal protein extraction buffer

Secondary antibodies

Background

Product citations

Selected references To be added when available, antibody released in June 2019.

Related products: ATG7 | Ubiquitin-like modifier-activating enzyme atg7

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
From 15 €
AS14 2769  |  Clonality: Polyclonal   |  Host: Rabbit  |  Reactivity...
278 €
Buy 2 items of this product for 206.00 €/items
Buy 3 items of this product for 190.00 €/items
AS09 607 Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
190 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
185 €