ATG8 | Autophagy-related protein
AS14 2769 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, A. madagascariensis, C. reinhardtii, H. lacustris, N. benthamiana, P. trichocarpa. S. lycopersicum

Data sheet | Product citations | Protocols | Customer reviews |
Product Information
Fragment of recombinant ATG8 from Chlamydomonas reinhardtii, UniProt: A8JB85
Reactivity
Ananas comosus, Brassica napus, Micromonas sp., Oryza sativa, Panicum hallii, Phoenix dactylifera, Physcomitrium patens, Pinus sitchensis, Solanum tuberosum, Volvox carteri
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Application examples




Reactant: Chlamydomonas reinhardtii (Green Alga)
Application: Western Blotting
Pudmed ID: 32765574
Journal: Front Plant Sci
Figure Number: 6B
Published Date: 2020-08-09
First Author: Chen, X. D., Liu, Y., et al.
Impact Factor: 5.435
Open PublicationEffects of different H2S donors or H2S biosynthesis inhibitors on cell autophagy capability of C. reinhardtii under MC-LR treatment. (A) Effects of H2S biosynthesis scavenger or inhibitor on MC-LR-induced expression of ATG1, AGT7, AGT8, and ATG101. Here, 3-d-old C. reinhardtii cells were treated with 100 nM MC-LR with or without H2S artificial donor NaHS (100??M), GYY4137 (GYY, 10??M), H2S scavenger hypotaurine (HY, 10??M), or H2S biosynthesis enzyme inhibitor aminooxyacetic acid (AOA, 1?mM) for 12 h, with transcriptional levels of ATG1, AGT7, AGT8, and ATG101 then measured by qRT-PCR analysis. Values are means ± SD of three biological replicates. (B) Effects of H2S biosynthesis scavenger or inhibitor on MC-LR-induced protein accumulation of ATG8. Here, 3-d-old C. reinhardtii cells were treated with 100 nM MC-LR with or without H2S artificial donor for 3 d, with protein accumulation of ATG8 measured by Western blotting using anti-ATG8 antibody. Anti-Tubulin was used as the loading control.
Additional information
This product can be sold containing ProClin if requested.
This antibody is recognizing 1 ng of recombinant CrATG8.
Antigen used to elicit this antibody is conserved from 70-80 % in following ATG protein from Arabidopsis thaliana: ATG8a UniProt: Q8LEM4 ATG8B UniProt: Q9XEB5 ATG8c UniProt: Q8S927 ATG8d UniProt: Q9SL04, ATG8e UniProt: Q8S926 ATG8f UniProt: Q8VYK7 and conserved below 70 % in: ATG8g UniProt: Q9LZZ9 ATG8h Uniprot: Q8S92
Important note:
For immunolocalization protocol, please inquire.
Background
Product citations
Li et al. (2021) Chloroplast proteotoxic stress-induced autophagy is involved in the degradation of chloroplast proteins in Chlamydomonas reinhardtii. Plant Cell Physiol. 2021 Feb 17:pcab029. doi: 10.1093/pcp/pcab029. Epub ahead of print. PMID: 33594417.
Li et al. (2021) The unfolded protein response plays dual roles in rice stripe virus infection through fine-tuning the movement protein accumulation. PLoS Pathog. 2021 Mar 4;17(3):e1009370. doi: 10.1371/journal.ppat.1009370. Epub ahead of print. PMID: 33662041.
Sun et al. (2021) Mechanistic insights into an atypical interaction between ATG8 and SH3P2 in Arabidopsis thaliana. Autophagy. 2021 Oct 17:1-17. doi: 10.1080/15548627.2021.1976965. Epub ahead of print. PMID: 34657568.
Mishra et al. (2021) Interplay between abiotic (drought) and biotic (virus) stresses in tomato plants. Mol Plant Pathol. 2021 Dec 30. doi: 10.1111/mpp.13172. Epub ahead of print. PMID: 34970822.
De Brasi-Velasco et al. (2021). Autophagy Is Involved in the Viability of Overexpressing Thioredoxin o1 Tobacco BY-2 Cells under Oxidative Conditions. Antioxidants. 2021; 10(12):1884. https://doi.org/10.3390/antiox10121884
Kazibwe et al. (2020). TOR mediates the autophagy response to altered nucleotide homeostasis in a ribonuclease mutant. J Exp Bot. 2020 Sep 9;eraa410.doi: 10.1093/jxb/eraa410.
Shull et al. (2019). Anatase TiO2 nanoparticles induce autophagy and chloroplast degradation in thale cress (Arabidopsis thaliana). Environ Sci Technol. 2019 Jul 29. doi: 10.1021/acs.est.9b01648.
Upadhyaya and Jagadeeshwar Rao (2019). Reciprocal regulation of photosynthesis and mitochondrial respiration by TOR kinase in Chlamydomonas reinhardtii. Plant Direct Volume 3, Issue 11.
Wojciechowska et al. (2018). Autophagy counteracts instantaneous cell death during seasonal senescence of the fine roots and leaves in Populus trichocarpa. BMC Plant Biol. 2018 Oct 29;18(1):260. doi: 10.1186/s12870-018-1439-6. (immunolocalization)
Chen et al. (2016). The role of nitric oxide signalling in response to salt stress in Chlamydomonas reinhardtii. Planta. 2016 Sep;244(3):651-69. doi: 10.1007/s00425-016-2528-0. Epub 2016 Apr 26.
Gorovits et al. (2016). Tomato yellow leaf curl virus confronts host degradation by sheltering in small/midsized protein aggregates. Virus Res. 2016 Feb 2;213:304-13. doi: 10.1016/j.virusres.2015.11.020. Epub 2015 Dec 1
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