ATG8A-I | Autophagy-related protein 8A-I isoforms

PRODUCT INFORMATION IN PDF

product information Background ATG8 (Autophagy-related protein) involved in degradation and recycling of intracellular components in a process of autophagy. Immunogen Part of recombinant ATG8A from Arabidopsis thaliana, UniProt:Q8LEM4,TAIR:AT4G21980 Host Rabbit Clonality Polyclonal Clone Purity Serum Format Lyophilized Quantity 50 µl Reconstitution For reconstitution add 50 µl of sterile water. Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications Western blot (WB) Related products AS14 2769 | anti-ATG8 | Autophagy-related protein, rabbit antibodies AS14 2769PRE | ATG8 | autophagy-related protein 8, pre-immune serum Plant and algal protein extraction buffer Secondary antibodies Additional information This product can be sold containing ProClin if requested.

application information
Recommended dilution		1 :4000 (WB)
Expected | apparent MW		13.6 kDa
Confirmed reactivity		Recombinant ATG8 A-I of Arabidopsis thaliana
Predicted reactivity
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information		This antibody is recognzing recombinant ATG8 isoforms (A to I) of Arabidopsis thaliana overexpressed with GST.
Selected references		To be added when available, antibody released in April 2016.

Application example



2 µg of respective recombinant Arabidopsis thaliana ATG8 isoform, denatured at 95C for 2 min. were separated on % SDS-PAGE and blotted 1h to nitrocellulose (GE10600003) using semi-dry transfer. Blots were blocked with 3% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 4 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:5 000 in for 1h at RT with agitation. The blot was washed as above and developed on film (GE28-9068-37) with Western Blotting Luminol Reagent (sc-2048). Exposure time was 1 second.

Courtesy of Dr. Steingrim Svenning, UiT, The Arctic University, Norway

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