ATG8A-I | Autophagy-related protein 8A-I isoforms
AS14 2811 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
DATA SHEET IN PDF| Data sheet | Product citations | Protocols | Add review |
Product Information
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
50 µl
Reconstitution
For reconstitution add 50 µl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB), Immunogold (IG)
Recommended dilution
1 :4000 (WB)
Expected | apparent MW
13.6 kDa
Reactivity
Confirmed reactivity
Recombinant ATG8 A-I of Arabidopsis thaliana
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Application examples
Application example
2 µg of respective recombinant Arabidopsis thaliana ATG8 isoform, denatured at 95C for 2 min. were separated on % SDS-PAGE and blotted 1h to nitrocellulose (GE10600003) using semi-dry transfer. Blots were blocked with 3% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 4 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:5 000 in for 1h at RT with agitation. The blot was washed as above and developed on film (GE28-9068-37) with high sensitivity chemiluminescent detection reagent. Exposure time was 1 second.
Courtesy of Dr. Steingrim Svenning, UiT, The Arctic University, Norway
2 µg of respective recombinant Arabidopsis thaliana ATG8 isoform, denatured at 95C for 2 min. were separated on % SDS-PAGE and blotted 1h to nitrocellulose (GE10600003) using semi-dry transfer. Blots were blocked with 3% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 4 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:5 000 in for 1h at RT with agitation. The blot was washed as above and developed on film (GE28-9068-37) with high sensitivity chemiluminescent detection reagent. Exposure time was 1 second.
Courtesy of Dr. Steingrim Svenning, UiT, The Arctic University, Norway
Additional information
Additional information
This product can be sold containing ProClin if requested.
This antibody is recognzing recombinant ATG8 isoforms (A to I) of Arabidopsis thaliana overexpressed with GST.
Background
Background
ATG8 (Autophagy-related protein) involved in degradation and recycling of intracellular components in a process of autophagy.
Product citations
Related products: ATG8A-I | Autophagy-related protein 8A-I isoforms
AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
This product can b...
This product can b...
From 25 €
AS19 4276 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis...
292 €
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items
AS19 4277 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis...
292 €
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
Off...
Off...
194 €
Agrisera AB Box 57, SE-911 21 Vännäs, SWEDEN | Phone: +46(0)935 33000 | Fax: +46(0)935 33044 | 
