ATG8A-I | Autophagy-related protein 8A-I isoforms

AS14 2811 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

ATG8A-I | Autophagy-related protein 8A-I isoforms

Product Information

Immunogen Part of recombinant ATG8A from Arabidopsis thaliana, UniProt:Q8LEM4,TAIR:AT4G21980

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB), Immunogold (IG)

Recommended dilution 1 :4000 (WB)

Expected | apparent MW 13,6 kDa

Reactivity

Confirmed reactivity Recombinant ATG8 A-I of Arabidopsis thaliana

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

2 µg of respective recombinant Arabidopsis thaliana ATG8 isoform, denatured at 95C for 2 min. were separated on % SDS-PAGE and blotted 1h to nitrocellulose (GE10600003) using semi-dry transfer. Blots were blocked with 3% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 4 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:5 000 in for 1h at RT with agitation. The blot was washed as above and developed on film (GE28-9068-37) with high sensitivity chemiluminescent detection reagent. Exposure time was 1 second.

Courtesy of Dr. Steingrim Svenning, UiT, The Arctic University, Norway

Reactant: Arabidopsis thaliana (Thale cress)

Application: Western Blotting

Pudmed ID: 29079776

Journal: Nat Commun

Figure Number: 5A

Published Date: 2017-10-27

First Author: Dong, Y., Silbermann, M., et al.

Impact Factor: 13.783

Open Publication

Autophagy is specifically induced by limited S-precursor supply for cysteine biosynthesis. a Autophagy induction in the shoot of serat tko and sir1-1 was determined by immunological detection of the canonical autophagy marker ATG8a and NBR1 with specific antisera. Lipidation of ATG8a (ATG8a-PE) (apparent size: 15–20?kDa) is essential for autophagosome formation and indicated by a significant shift during electrophoresis. NBR1 is a cargo receptor for selective autophagy and consequently degraded in autophagic bodies (D-NBR1) (apparent size: 50–75?kDa). b Level of ATG8a-PE and D-NBR1 shown in a were quantified (n?=?3, mean?±?s.e.m., one-way ANOVA, *p?

Reactant: Arabidopsis thaliana (Thale cress)

Application: Western Blotting

Pudmed ID: 29079776

Journal: Nat Commun

Figure Number: 5D

Published Date: 2017-10-27

First Author: Dong, Y., Silbermann, M., et al.

Impact Factor: 13.783

Open Publication

Additional information

Additional information This product can be sold containing ProClin if requested

This antibody is recognzing recombinant ATG8 isoforms (A to I) of Arabidopsis thaliana overexpressed with GST.

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Background

Background ATG8 (Autophagy-related protein) involved in degradation and recycling of intracellular components in a process of autophagy.

Product citations

Selected references Fan et al. (2019). Dual Role for Autophagy in Lipid Metabolism in Arabidopsis. Plant Cell. 2019 Jul;31(7):1598-1613. doi: 10.1105/tpc.19.00170. (Immunogold)
Dong et al. (2017). Sulfur availability regulates plant growth via glucose-TOR signaling. Nat Commun. 2017 Oct 27;8(1):1174. doi: 10.1038/s41467-017-01224-w.