IKP

MTP1 | vacuolar Zn2+/H+ antiporter

Product no: AS09 485

AS09 485 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Nicotiana tabacum

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana MTP1 UniProt:Q9ZT63, TAIR:At2g46800

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 100 µl
    Reconstitution: For reconstitution add 100 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Western blot (WB)
    Recommended dilution: 1 : 8000 (ELISA), 1 : 1000 (WB)
    Expected | apparent MW:

    43.8 | 43 kDa (Arabidopsis thaliana)

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Nicotiana tabacum
    Predicted reactivity: Brassica sp. , Noccaea fendleri, Thlaspi caerulescens
    Species of your interest not listed? Contact us
    Not reactive in: Hordeum vulgare, Solanaceae sp.
  • Application Examples
  • Application example

     

    western blot using anti-AtMTP1 antibodies

    Sample of a vector (1) and vacuolar membrane fraction of yeast cells expressing AtMTP1 (2) separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-AtMTP1 antibodies (AS09 485, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

  • Additional Information
  • Additional information:

    0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

    MTP1 protein is of low abundance in plant tissues.

    Additional information (application): Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel
  • Background
  • Background:

    MTP1 is mediating zinc accumulation in roots and resistance to zinc. Is involved in sequestering of zinc from cytoplasm into vacuole in conditions of its excess.

    Alternative names: Zinc transporter ZAT-1, ZAT1p, 43.8 | metal tolerance protein 1

  • Product Citations
  • Selected references: Vera-Estrella et al. (2017). Cadmium and zinc activate adaptive mechanisms in Nicotiana tabacum similar to those observed in metal tolerant plants. Planta. 2017 Apr 28. doi: 10.1007/s00425-017-2700-1.
    Kawachi et al. (2008). Deletion of a histidine-rich loop of AtMTP1, a vacuolar Zn(2+)/H(+) antiporter of Arabidopsis thaliana, stimulates the transport activity. J.Biol. Chem. 13:8374-8383.
    Kobae et al. (2004). Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis. Plant Cell Physiol. 12:1749-1758.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection



    Method for isolation of plant vacuolar membranes

    method for vacuolar membrane isolation

    Courtesy of Dr. Masayoshi Maeshima, Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences Nagoya University Nagoya, Japan

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