MTP1 | vacuolar Zn2+/H+ antiporter

AS09 485 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Nicotiana tabacum

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana MTP1 Q9ZT63, At2g46800

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1 : 8000 (ELISA), 1 : 1000 (WB)

Expected | apparent MW

43.8 | 43 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Nicotiana tabacum

Predicted reactivity Brassica sp. , Noccaea fendleri, Thlaspi caerulescens

Not reactive in Members of Solanaceae

Application examples

Application example

western blot using anti-AtMTP1 antibodies

Sample of a vector (1) and vacuolar membrane fraction of yeast cells expressing AtMTP1 (2) separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-AtMTP1 antibodies (AS09 485, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

MTP1 protein is of low abundance in plant tissues.

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Related products

collection of antibodies to plant tonoplast membrane proteins

AS09 474 | anti-AtMTP4 | metal tolerance protein B

Plant protein extraction buffer

Secondary antibodies

Background

MTP1 is mediating zinc accumulation in roots and resistance to zinc. Is involved in sequestering of zinc from cytoplasm into vacuole in conditions of its excess. Alternative names: Zinc transporter ZAT-1, ZAT1p, 43.8 | metal tolerance protein 1

Product citations

Selected references Vera-Estrella et al. (2017). Cadmium and zinc activate adaptive mechanisms in Nicotiana tabacum similar to those observed in metal tolerant plants. Planta. 2017 Apr 28. doi: 10.1007/s00425-017-2700-1.
Kawachi et al. (2008). Deletion of a histidine-rich loop of AtMTP1, a vacuolar Zn(2+)/H(+) antiporter of Arabidopsis thaliana, stimulates the transport activity. J.Biol. Chem. 13:8374-8383.
Kobae et al. (2004). Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis. Plant Cell Physiol. 12:1749-1758.