MTP1 | vacuolar Zn2+/H+ antiporter
AS09 485 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Nicotiana tabacum
|Data sheet||Product citations||Protocols||Add review|
43.8 | 43 kDa (Arabidopsis thaliana)
Species of your interest not listed? Contact us
Sample of a vector (1) and vacuolar membrane fraction of yeast cells expressing AtMTP1 (2) separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-AtMTP1 antibodies (AS09 485, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.
MTP1 protein is of low abundance in plant tissues.
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
MTP1 is mediating zinc accumulation in roots and resistance to zinc. Is involved in sequestering of zinc from cytoplasm into vacuole in conditions of its excess.
Alternative names: Zinc transporter ZAT-1, ZAT1p, 43.8 | metal tolerance protein 1
Related products: MTP1 | vacuolar Zn2+/H+ antiporter
This product can b...