IKP

AtpF | CF0I subunit of ATP synthase

Product no: AS10 1604
AS10 1604 | Clonality: Polyclonal | Host: Rabbit | Reactivity:A. thaliana, S. oleracea, Ch. reinhardtii
413
Add to cart
Delivery:  7-10 business days
  • Product Info
  • Immunogen: Isolated CF0I subunit of the chloroplast ATP synthase complex of Arabidopsis thaliana, UniProt UniProt::P56759, TAIR: ATCG00130
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Liquid
    Quantity: 100 l
    Storage: Store at short-term 4C, Long-term -20. Repeated freezing and thawing is not recommended. It ontains 0,01% sodium azide.
    Tested applications: Blue Native PAGE (BN-PAGE), Western blot (WB)
    Recommended dilution: 1 : 5000 (BN-PAGE), (WB)
    Expected | apparent MW:

    21 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Spinacia oleracea, Ulva prolifera
    Predicted reactivity: Cannabis sativa, Higher plants, Phaseolus vulgaris, Pisum sativum
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    western blot using anti-AtpF antibodies

    20 µg of chloroplast fraction from Arabidopsis thaliana (1) and Spinacia oleracea (2)  were separated on 12  % SDS-PAGE  and blotted 1h to PVDF. Blots were blocked with 2 % non-fat milk powder in 1xTBS-T  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions.
  • Additional Information
  • Additional information: This product can be sold containing proClin if requested
  • Background
  • Background: The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
  • Reviews:
  • To leave a review, you should have previously purchased this product and to your customer profile.

    This product doesn't have any reviews.

Accessories

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

201 €
Add to cart
Info

AS09 607 |  Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

206 €
Add to cart
Info
AS10 1583  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, N. tabacum, S. oleracea, C. reinhardtii
413 €
Add to cart
Info
img missing
AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

Now available only as separate products:
1 x AS16 ECL-N
1 x AS16 ECL-S

This product can be purchased in 3 different volumes:

AS16 ECL-SN-10, 10 ml. Trial size limited to one per customer

AS16 ECL-SN, 100 ml

AS16 ECL-SN-1L, 1L

Choose the appropriate volume in the drop down menu to the right

From 26 €
Close