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AtpF | CF0I subunit of ATP synthase
Product no: AS10 1604
AS10 1604 | Clonality: Polyclonal | Host: Rabbit | Reactivity:A. thaliana, S. oleracea, Ch. reinhardtii
413
€
Delivery:
7-10 business days
- Product Info
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Immunogen: Isolated CF0I subunit of the chloroplast ATP synthase complex of Arabidopsis thaliana, UniProt UniProt::P56759, TAIR: ATCG00130
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Liquid Quantity: 100 µl Storage: Store at short-term 4°C, Long-term -20°. Repeated freezing and thawing is not recommended. It ontains 0,01% sodium azide. Tested applications: Blue Native PAGE (BN-PAGE), Western blot (WB) Recommended dilution: 1 : 5000 (BN-PAGE), (WB) Expected | apparent MW: 21 kDa
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Spinacia oleracea, Ulva prolifera
Predicted reactivity: Cannabis sativa, Higher plants, Phaseolus vulgaris, Pisum sativum
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Application example 
20 µg of chloroplast fraction from Arabidopsis thaliana (1) and Spinacia oleracea (2) were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 2 % non-fat milk powder in 1xTBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. - Additional Information
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Additional information: This product can be sold containing proClin if requested - Background
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Background: The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection
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Now available only as separate products:
1 x AS16 ECL-N
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Now available only as separate products:
1 x AS16 ECL-N
1 x AS16 ECL-S
This product can be purchased in 3 different volumes:
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Choose the appropriate volume in the drop down menu to the right
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