AtpF | CF0I subunit of ATP synthase
AS10 1604 | Clonality: Polyclonal | Host: Rabbit | Reactivity:A. thaliana, S. oleracea, Ch. reinhardtii
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Product Information
Immunogen
Isolated CF0I subunit of the chloroplast ATP synthase complex of Arabidopsis thaliana, UniProt UniProt::P56759, TAIR: ATCG00130
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Liquid
Quantity
100 ĩl
Storage
Store at short-term 4°C, Long-term -20°. Repeated freezing and thawing is not recommended. It ontains 0,01% sodium azide.
Tested applications
Blue Native PAGE (BN-PAGE), Western blot (WB)
Recommended dilution
1 : 5000 (BN-PAGE), (WB)
Expected | apparent MW
21 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana, Chlamydomonas reinhardtii, Spinacia oleracea, Ulva prolifera
Predicted reactivity
Cannabis sativa, Higher plants, Phaseolus vulgaris, Pisum sativum
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
Application example
20 µg of chloroplast fraction from Arabidopsis thaliana (1) and Spinacia oleracea (2) were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 2 % non-fat milk powder in 1xTBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions.
20 µg of chloroplast fraction from Arabidopsis thaliana (1) and Spinacia oleracea (2) were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 2 % non-fat milk powder in 1xTBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions.
Additional information
Additional information
This product can be sold containing proClin if requested
Background
Background
The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient.
Product citations
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