AtpF | CF0I subunit of ATP synthase

AS10 1604 | Clonality: Polyclonal | Host: Rabbit | Reactivity:A. thaliana, S. oleracea, Ch. reinhardtii

Product Information

Immunogen Isolated CF₀I subunit of the chloroplast ATP synthase complex of Arabidopsis thaliana, UniProt UniProt::P56759, TAIR: ATCG00130

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Liquid

Quantity 100 µl

Storage Store at short-term 4°C. Long-term -20°. Repeated freezing and thawing is not recommended. Solution contains 0.01% sodium azide as preservative.

Tested applications Blue Native PAGE (BN-PAGE), Western blot (WB)

Recommended dilution 1 : 5000 (BN-PAGE), (WB)

Expected | apparent MW

21 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Chlamydomonas reinhardtii, Spinacia oleracea, Ulva prolifera

Predicted reactivity Cannabis sativa, Higher plants, Phaseolus vulgaris, Pisum sativum
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

western blot using anti-AtpF antibodies

20 µg of chloroplast fraction from Arabidopsis thaliana (1) and Spinacia oleracea (2) were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 2 % non-fat milk powder in 1xTBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions.

Additional information

This product can be sold containing proClin if requested.

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Secondary antibodies

Background

Background The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient.