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AtpG | ATPsynthase subunit II b' (chloroplastic)

AS09 457 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii

AtpG | ATPsynthase subunit II b' (chloroplastic) in the group Antibodies Plant/Algal  / Photosynthesis  / ATP synthase at Agrisera AB (Antibodies for research) (AS09 457)
AtpG | ATPsynthase subunit II b' (chloroplastic)



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Product Information

Immunogen

KLH-conjugated mix of synthetic peptide derived from Arabidopsis thaliana AtpG Q0WMW8, At4g32260 and Chlamydomonas reinhardtii ATP synthase subunit b' A8J785

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 10 000 (WB)
Expected | apparent MW

22.4 kDa (Chlamydomonas reinhardtii) 15.9 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Chlamydomonas reinhardtii
Predicted reactivity

Algae, Oryza sativa, Sorghum bicolor,  Zea mays, Volvox carteri


Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples

Application example

western blot detection using anti-AtpG subunit II antibodies

10 ug of chlorophyll/well of Chlamydomonas reinhardtii total cell extract (1), Chlamydomonas reinhardtii subunit II deletion mutant thylakoid membrane fraction (2), Arabidospsis thaliana thylakoid membrane fraction (3), were separated on 12-18% acrylamide-8M urea gel and blotted to nitrocellulose membrane. Filters were blocked 1 h with 5% dry milk in 1 x PBS and probed with anti-ATP synthase subunit gamma antibody (AS09 457, 1: 10 000, 1h) and secondary HRP-conjugated anti-rabbit antibody (1: 10 000, 1 h) in 1 x PBS containing 5% dry milk. All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent, exposure time was 1 min.

Arabidopsis membrane preparation has been done according to Lezhneva et al. (2008) A novel pathway of cytochrome c biogenesis is involved in the assembly of the cytochrome b6f complex in arabidopsis chloroplasts. J Biol. Chem., 283:24608-24616 and Chlamydomonas membranes were prepared according to Chua & Bennoun (1975) Thylakoid membrane polypeptides of Chlamydomonas reinhardtii: wild-type and mutant strains deficient in photosystem II reaction center. PNAS 72:2175-2179

Courtesy Dr. Yves Choquet, CNRS, France

Additional information

Additional information This product can be sold containing ProClin if requested

Antibody reactivity for Arabidopsis thaliana has been confirmed on membrane fraction.

Related products

Background

Background

ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.

Alternative names: ATPase subunit II, ATP synthase F(0) sector subunit b'

Product citations

immunogen:

KLH-conjugated mix of synthetic peptide derived from Arabidopsis thaliana AtpG Q0WMW8, At4g32260 and Chlamydomonas reinhardtii ATP synthase subunit b' A8J785

Host: Rabbit
Clonality: Polyclonal
Purity: Serum
Format: Lyophilized
Quantity: 100 ĩl
Reconstitution: For reconstitution add 100 ĩl of sterile water
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications: Western blot (WB)
recommended dilution: 1 : 10 000 (WB)
Expected | apparent MW:

22.4 kDa (Chlamydomonas reinhardtii) 15.9 kDa (Arabidopsis thaliana)

Confirmed reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii
predicted reactivity:

Algae, Oryza sativa, Sorghum bicolor,  Zea mays, Volvox carteri


Species of your interest not listed? Contact us
not reactive in: No confirmed exceptions from predicted reactivity are currently known
Picture (footer):

Application example

western blot detection using anti-AtpG subunit II antibodies

10 ug of chlorophyll/well of Chlamydomonas reinhardtii total cell extract (1), Chlamydomonas reinhardtii subunit II deletion mutant thylakoid membrane fraction (2), Arabidospsis thaliana thylakoid membrane fraction (3), were separated on 12-18% acrylamide-8M urea gel and blotted to nitrocellulose membrane. Filters were blocked 1 h with 5% dry milk in 1 x PBS and probed with anti-ATP synthase subunit gamma antibody (AS09 457, 1: 10 000, 1h) and secondary HRP-conjugated anti-rabbit antibody (1: 10 000, 1 h) in 1 x PBS containing 5% dry milk. All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent, exposure time was 1 min.

Arabidopsis membrane preparation has been done according to Lezhneva et al. (2008) A novel pathway of cytochrome c biogenesis is involved in the assembly of the cytochrome b6f complex in arabidopsis chloroplasts. J Biol. Chem., 283:24608-24616 and Chlamydomonas membranes were prepared according to Chua & Bennoun (1975) Thylakoid membrane polypeptides of Chlamydomonas reinhardtii: wild-type and mutant strains deficient in photosystem II reaction center. PNAS 72:2175-2179

Courtesy Dr. Yves Choquet, CNRS, France

additional information: This product can be sold containing ProClin if requested
additional information (application):

Antibody reactivity for Arabidopsis thaliana has been confirmed on membrane fraction.

background:

ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.

Alternative names: ATPase subunit II, ATP synthase F(0) sector subunit b'

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