showForm anti-AtpG | ATPsynthase subunit IIB' antibodies

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AtpG | ATPsynthase subunit II b' (chloroplastic)

AS09 457 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii

AtpG | ATPsynthase subunit II b' (chloroplastic) in the group Antibodies for Plant/Algal  / Photosynthesis  / ATP synthase at Agrisera AB (Antibodies for research) (AS09 457)

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Product Information

Immunogen

KLH-conjugated mix of synthetic peptide derived from Arabidopsis thaliana AtpG Q0WMW8, At4g32260 and Chlamydomonas reinhardtii ATP synthase subunit b' A8J785

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 10 000 (WB)
Expected | apparent MW

22.4 kDa (Chlamydomonas reinhardtii) 15.9 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Chlamydomonas reinhardtii
Predicted reactivity

Algae, Oryza sativa, Sorghum bicolor,  Zea mays, Volvox carteri

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

western blot detection using anti-AtpG subunit II antibodies

10 ug of chlorophyll/well of Chlamydomonas reinhardtii total cell extract (1), Chlamydomonas reinhardtii subunit II deletion mutant thylakoid membrane fraction (2), Arabidospsis thaliana thylakoid membrane fraction (3), were separated on 12-18% acrylamide-8M urea gel and blotted to nitrocellulose membrane. Filters were blocked 1 h with 5% dry milk in 1 x PBS and probed with anti-ATP synthase subunit gamma antibody (AS09 457, 1: 10 000, 1h) and secondary HRP-conjugated anti-rabbit antibody (1: 10 000, 1 h) in 1 x PBS containing 5% dry milk. All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent, exposure time was 1 min.

Arabidopsis membrane preparation has been done according to Lezhneva et al. (2008) A novel pathway of cytochrome c biogenesis is involved in the assembly of the cytochrome b6f complex in arabidopsis chloroplasts. J Biol. Chem., 283:24608-24616 and Chlamydomonas membranes were prepared according to Chua & Bennoun (1975) Thylakoid membrane polypeptides of Chlamydomonas reinhardtii: wild-type and mutant strains deficient in photosystem II reaction center. PNAS 72:2175-2179

Courtesy Dr. Yves Choquet, CNRS, France

Additional information

Additional information This product can be sold containing ProClin if requested.

Antibody reactivity for Arabidopsis thaliana has been confirmed on membrane fraction.

Related products

Related products

AS08 370 | Anti-ATP synthase, whole enzyme, rabbit antibodies

AS08 304 | Anti-ATP synthase subunit alpha, rabbit antibodies

AS03 030 | Anti-ATP synthase subunit beta, hen antibodies

AS05 071 | Anti-ATP synthase subunit c, rabbit antibodies

AS08 312 | Anti-AtpC | gamma subunit of ATP synthase, rabbit antibodies

AS07 260 | Anti-H+ATPase | plasma membrane H+ATPase, rabbit antibodies

Background

Background

ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.

Alternative names: ATPase subunit II, ATP synthase F(0) sector subunit b'

Product citations

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