AUX1 | Auxin transporter protein 1 (goat antibody)

AS16 3957 | Clonality: Polyclonal | Host: Goat | Reactivity: Arabidopsis thaliana

AUX1 | Auxin transporter protein 1 (goat antibody) in the group Antibodies for Plant/Algal  / Hormones / Biosynthesis/regulation at Agrisera AB (Antibodies for research) (AS16 3957)


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Product Information

Immunogen KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana AUX1. UniProt: Q96247, TAIR: AT2G38120
Host Goat
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.

Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Immunolocalization (IL), Western blot (WB)
Recommended dilution 1: 250 - 1: 700 (IL), 1: 5000 (WB) on recombinant AUX1


Confirmed reactivity Arabidopsis thaliana (recombinant AUX1)
Predicted reactivity Arabidopsis thaliana
Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Application examples

Application examples Application example

Western blot using anti-AUX1 goat antibody

Arabidopsis thaliana
AUX1 was expressed in insect cells using baculovirus infection. Expression was driven by the strong polyhedrin promoter. Cultures of Sf9 cells (20 ml, approx. 2 x 107 cells) were infected with virus at multiplicities of infection (MOI) of 0.1, 1 and 2.5. 2 mL samples were harvested by centrifugation after 3 days, lysed (20 mM Tris/HCl, pH 7.4, 200 mM NaCl, 1 mM EDTA, 1% Tween 20, protease inhibitors and DNAseI) at 4°C for 30 min, sonicated (3 x 5 s pulses) and centrifuged. Cleared supernatant samples (whole cell lysates) were run on SDS-PAGE using 8 – 13% acrylamide gradient gels. After transfer to PVDF, the membrane was blocked with TBS-Tween with 10% milk powder overnight. Primary antibodies were applied at 1:5000 dilution in TBS-Tween with 5% milk powder for one hour at room temperature, washed x3 in TBS-Tween for 10 mins each and secondary antibodies (e.g. AS09 605 rabbit anti-goat HRP conjugated, Agrisera) applied diluted 1:10 000 as above. After washing as above, development was by ECL using Millipore Immobilon Transfer reagent for 2 mins and the image captured by ImageQuant. As negative controls, samples of non-infected cells (NC) were run alongside the AUX1 extracts, as well as protein size markers (Mr).

The complete fusion protein (enhanced GFP-AtAUX1) was detected at close to 60 kDa A breakdown or truncated band was also seen at a little over 40 kDa.

Courtesty of Prof Richard Napier, University of Warwick, UK

Immunolocalization using anti-AUX1 antibodies

Whole mount tissue immunochemistry of sections of protophloems of Arabidopsis thaliana root tips.
Fixation: 4% PFA in MTSB Buffer pH 6.9 Vacum 2x5’ and 1x40’
Blocking: BSA in MTSB (0.4g in 20ml), As a secondary antibody Alexafluor 546 donkey, anti-goat, concentration 1:500.

Comparison between the two alleles: aux1-21 has a frame shift after exon 5, whereas aux1-2 only has an amino acid substitution and expression is checked in the protophloem.

Courtesy Ana Cecilia Aliaga Fandino ,Hardtke lab, University of Lausanne, Switzerland

Additional information

Reactivity of this antibody on endogenous AUX1 remains to be determined.

Related products

Related products Collection of antibodies to auxins and proteins involved in auxin metabolism

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Background AUX1 (Auxin transporter protein 1) is a carrier protein involved in proton-driven auxin influx. Synthesized in developing leaves and transpported to tips. Involved in lateral root formation, trichoblast polarization and root hair elongation. Required for gravitropism and thigmotropism, especially in roots, by modulating responses to auxin, ethylene and cytokinins. Needed for ammonium-mediated root-growth inhibition. Alternative names: AUX1, AUXIN RESISTANT 1, WAV5, WAVY ROOTS 5, PIR1, MAP1, MODIFIER OF ARF7/NPH4 PHENOTYPES 1, Auxin influx carrier protein 1, Polar auxin transport inhibitor-resistant protein 1.

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