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BC2-tag

AS20 4446   | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: BC2-tag

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BC2-tag in the group Tag Antibodies / GAL4 / GUS / LUC / others at Agrisera AB (Antibodies for research) (AS20 4446)

DATA SHEET IN PDF

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Product Information

Immunogen KLH-conjugated peptide derived from 
recombinat protein of...expressed in E.coli
native, purified protein from
Host

Rabbit
Chicken
Goat
Mouse

Clonality Polyclonal
Purity Serum
Affinity purified serum, in PBS pH 7.4
Format Lyophilized
Liquid
Quantity µg or µl
Reconstitution For reconstitution add  µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example





µg of total protein extracted freshly from    with write exact buffer components ..... and denatured with....at °C or 70°C for 2-5 min.... were separated on  % SDS-PAGE  and blotted 1h to PVDF/nitrocellulose using wet, semi-dry or dry transfer. Blot was blocked with % milk or % BSA  for 1h/RT or 4°C/ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with agitation in TBS-T or ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in  for 1h/RT with agitation. The blot was washed as above and developed for   min with   name and supplier. Exposure time was.....seconds.

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Additional information

Related products

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Background

Background Background info.

Product citations

Selected references To be added when available, antibody available in

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