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Cp2 | Cysteine protease

AS16 3110 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Carica papaya

Cp2 | Cysteine protease  in the group Antibodies Plant/Algal  / Environmental Stress / Pathogen attack at Agrisera AB (Antibodies for research) (AS16 3110)
Cp2 | Cysteine protease



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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

KLH-conjugated peptide derived from cysteine protease sequence of Carica papaya UniProt: H6USN1

Host Rabbit
Clonality Polyclonal
Purity Total IgG. Protein G purified in PBS pH 7.4.
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 51,4 | 40 kDa

Reactivity

Confirmed reactivity Carica papaya
Predicted reactivity Cajanus cajan, Cicer arietinum, Cucumis sativus, Glycine soja, Gossypium hirsutum, Medicago truncatula, Phaseolus vulgaris, Trifolium pratense, Vicia sativa, Vigna radiata var. radiata
Species of your interest not listed? Contact us
Not reactive in

Arabidopsis thaliana

Application examples

Application examples Application example

western blot using anti-C protease 2 antibodies


Total proteins from Carica papaya cultivar Eksotika and MARDI Purple leaves were extracted using pre-cooled extraction buffer containing 50 mM phosphate buffer pH7 and protease inhibitor cocktail. 60 µg of total protein was blotted 1 h to PVDF using semi-dry transfer cell (BioRad, USA). Blots were blocked with 3% milk in PBST for overnight at 4°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 in 1% BSA/PBST for 3h at 4°C with agitation. The antibody solution was decanted and the blot was washed 3 times for 5 min each in PBS at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602, Agrisera) diluted to 1:20 000 in 1% BSA/PBST for 2 h at RT with agitation. The blot was washed as above and stained with Amplified Opti-4CN Substrate kit (BioRad, USA).
Apparent MW of Cp2 is 40 kDa.

Courtesy of Suhaina Supian, Malaysian Agricultural Research and Development Institute, Malaysia

Additional information

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Background

Background

Cysteine protease Cp2 shows proteolytic activity and is involved in programmed cell death in plant during pathogen infection.

Product citations

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