Cp2 | Cysteine protease
AS16 3110 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Carica papaya
|Recommended dilution||1 : 1000 (WB)|
|Expected | apparent MW||
51.4 | 40 kDa
|Confirmed reactivity||Carica papaya|
|Not reactive in||
|Selected references||to be added when available, antibody released in January 2016.|
Total proteins from Carica papaya cultivar Eksotika and MARDI Purple leaves were extracted using pre-cooled extraction buffer containing 50 mM phosphate buffer pH7 and protease inhibitor cocktail. 60 µg of total protein was blotted 1 h to PVDF using semi-dry transfer cell (BioRad, USA). Blots were blocked with 3% milk in PBST for overnight at 4°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 in 1% BSA/PBST for 3h at 4°C with agitation. The antibody solution was decanted and the blot was washed 3 times for 5 min each in PBS at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1:20 000 in 1% BSA/PBST for 2 h at RT with agitation. The blot was washed as above and stained with Amplified Opti-4CN Substrate kit (BioRad, USA).
Apparent MW of Cp2 is 40 kDa.
Courtesy of Suhaina Supian, Malaysian Agricultural Research and Development Institute, Malaysia
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