Anti-Cp2 | Cysteine protease

Product no: AS16 3110

AS16 3110 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Carica papaya

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  • Product Info
  • Immunogen:

    KLH-conjugated peptide derived from cysteine protease sequence of Carica papaya UniProt: H6USN1

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Total IgG. Protein G purified in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 µl
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 51.4 | 40 kDa
  • Reactivity
  • Confirmed reactivity: Carica papaya
    Predicted reactivity: Cajanus cajan, Cicer arietinum, Cucumis sativus, Glycine soja, Gossypium hirsutum, Medicago truncatula, Phaseolus vulgaris, Trifolium pratense, Vicia sativa, Vigna radiata var. radiata

    Species of your interest not listed? Contact us
    Not reactive in:

    Arabidopsis thaliana

  • Application Examples
  • Application example

    western blot using anti-C protease 2 antibodies

    Total proteins from Carica papaya cultivar Eksotika and MARDI Purple leaves were extracted using pre-cooled extraction buffer containing 50 mM phosphate buffer pH7 and protease inhibitor cocktail. 60 µg of total protein was blotted 1 h to PVDF using semi-dry transfer cell (BioRad, USA). Blots were blocked with 3% milk in PBST for overnight at 4°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 in 1% BSA/PBST for 3h at 4°C with agitation. The antibody solution was decanted and the blot was washed 3 times for 5 min each in PBS at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602, Agrisera) diluted to 1:20 000 in 1% BSA/PBST for 2 h at RT with agitation. The blot was washed as above and stained with Amplified Opti-4CN Substrate kit (BioRad, USA).
    Apparent MW of Cp2 is 40 kDa.

    Courtesy of Suhaina Supian, Malaysian Agricultural Research and Development Institute, Malaysia
  • Background
  • Background:

    Cysteine protease Cp2 shows proteolytic activity and is involved in programmed cell death in plant during pathogen infection.

  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts
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AS16 ECL-S | extreme low femtogram detection  |  Limited stock

This product can be purchased in 3 different volumes:

AS16 ECL-S-10, 10 ml. Trial size limited to one per customer

AS16 ECL-S-100, 100 ml

Choose the appropriate volume in the drop down menu to the right

From 17 €