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Anti-Cp2 | Cysteine protease
Product no: AS16 3110
AS16 3110 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Carica papaya
329
€
Customer reviews
Delivery:
3-6 business days
- Product Info
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Immunogen: KLH-conjugated peptide derived from cysteine protease sequence of Carica papaya UniProt: H6USN1
Host: Rabbit Clonality: Polyclonal Purity: Total IgG. Protein G purified in PBS pH 7.4. Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 51.4 | 40 kDa - Reactivity
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Confirmed reactivity: Carica papaya Predicted reactivity: Cajanus cajan, Cicer arietinum, Cucumis sativus, Glycine soja, Gossypium hirsutum, Medicago truncatula, Phaseolus vulgaris, Trifolium pratense, Vicia sativa, Vigna radiata var. radiata
Species of your interest not listed? Contact usNot reactive in: Arabidopsis thaliana
- Application Examples
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Application example 
Total proteins from Carica papaya cultivar Eksotika and MARDI Purple leaves were extracted using pre-cooled extraction buffer containing 50 mM phosphate buffer pH7 and protease inhibitor cocktail. 60 µg of total protein was blotted 1 h to PVDF using semi-dry transfer cell (BioRad, USA). Blots were blocked with 3% milk in PBST for overnight at 4°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 in 1% BSA/PBST for 3h at 4°C with agitation. The antibody solution was decanted and the blot was washed 3 times for 5 min each in PBS at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602, Agrisera) diluted to 1:20 000 in 1% BSA/PBST for 2 h at RT with agitation. The blot was washed as above and stained with Amplified Opti-4CN Substrate kit (BioRad, USA).
Apparent MW of Cp2 is 40 kDa.
Courtesy of Suhaina Supian, Malaysian Agricultural Research and Development Institute, Malaysia - Background
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Background: Cysteine protease Cp2 shows proteolytic activity and is involved in programmed cell death in plant during pathogen infection.
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts - Reviews:
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