CCS | Chloroplastic copper chaperone for SOD

Product no: AS07 219

AS07 219 |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana,  B. juncea, Z. mays, O. sativa

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  • Product Info
  • Immunogen:

    The antibody was raised to the Arabidopsis thaliana full length CCs protein Q4ZJI5,  fused to His-tag. Overexpressed using pET28a vector in Bl21 E. coli (Cd+) and purified using His-column. After purification, the His tag was cleaved using thrombin and then theprotein was purified using Resource-Q column connected to HPLC.

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 200 l
    Reconstitution: For reconstitution add 200 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW:

    33.8 | 37 kDa (Arabidopsis thaliana)

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Brassica juncea, Oryza sativa, Zea mays
    Predicted reactivity: Glycne max, Hordeum vulgare, Solanum tuberosum
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Background
  • Background:

    Copper is an essential element for function of chloroplasts. It is a co-factor for superoxide dismutase (SOD) and for plastocyanin. Copper is transported to chloroplasts with a help of Copper Chaperones, which carry out the delivery of copper from transporters to targets. In the last step chaperone binds to a copper dependent enzyme and inserts the copper ions into its active site. The Arabidopsis thaliana gene AtCCS encodes a copper chaperone for SOD. The AtCCS protein was localized to chloroplasts where it may supply copper to the stromal Cu/ZnSOD.

  • Product Citations
  • Selected references: Speth et al. (2013). RACK1 scaffold proteins influence miRNA abundance in Arabidopsis. Plant J. Aug 13.
    Abdel-Ghany et al (2005). .AtCCS is a functional homolog of the yeast copper chaperone Ccs1/Lys7.  FEBS Lett. 11:2307-12.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
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    Number of reviews: (1)
    karl | 2013-03-20
    0 | 0
    great

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