CCS | Chloroplastic copper chaperone for SOD

Name: CCS | Chloroplastic copper chaperone for SOD
SKU: AS07 219
Price: 302 EUR
Availability: InStock
Rating: 5 (1 reviews)

AS07 219 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. juncea, Z. mays, O. sativa

CCS | Chloroplastic copper chaperone for SOD in the group Antibodies Plant/Algal / Environmental Stress / Heavy metal stress at Agrisera AB (Antibodies for research) (AS07 219)

CCS | Chloroplastic copper chaperone for SOD

Product Information

Immunogen

The antibody was raised to the Arabidopsis thaliana full length CCs protein Q4ZJI5, fused to His-tag. Overexpressed using pET28a vector in Bl21 E. coli (Cd+) and purified using His-column. After purification, the His tag was cleaved using thrombin and then theprotein was purified using Resource-Q column connected to HPLC.

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 200 µl

Reconstitution For reconstitution add 200 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

33.8 | 37 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Brassica juncea, Oryza sativa, Zea mays

Predicted reactivity Glycne max, Hordeum vulgare, Solanum tuberosum
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Background

Copper is an essential element for function of chloroplasts. It is a co-factor for superoxide dismutase (SOD) and for plastocyanin. Copper is transported to chloroplasts with a help of Copper Chaperones, which carry out the delivery of copper from transporters to targets. In the last step chaperone binds to a copper dependent enzyme and inserts the copper ions into its active site. The Arabidopsis thaliana gene AtCCS encodes a copper chaperone for SOD. The AtCCS protein was localized to chloroplasts where it may supply copper to the stromal Cu/ZnSOD.

Product citations

Selected references Speth et al. (2013). RACK1 scaffold proteins influence miRNA abundance in Arabidopsis. Plant J. Aug 13.
Abdel-Ghany et al (2005). .AtCCS is a functional homolog of the yeast copper chaperone Ccs1/Lys7. FEBS Lett. 11:2307-12.

immunogen:	The antibody was raised to the Arabidopsis thaliana full length CCs protein Q4ZJI5, fused to His-tag. Overexpressed using pET28a vector in Bl21 E. coli (Cd+) and purified using His-column. After purification, the His tag was cleaved using thrombin and then theprotein was purified using Resource-Q column connected to HPLC.
Reconstitution:	For reconstitution add 200 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	200 µl
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 1000 (WB)
calculated \| apparent molecular mass [kDa]:	33.8 \| 37 kDa (Arabidopsis thaliana)
Confirmed reactivity:	Arabidopsis thaliana, Brassica juncea, Oryza sativa, Zea mays
predicted reactivity:	Glycne max, Hordeum vulgare, Solanum tuberosum Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
background:	Copper is an essential element for function of chloroplasts. It is a co-factor for superoxide dismutase (SOD) and for plastocyanin. Copper is transported to chloroplasts with a help of Copper Chaperones, which carry out the delivery of copper from transporters to targets. In the last step chaperone binds to a copper dependent enzyme and inserts the copper ions into its active site. The Arabidopsis thaliana gene AtCCS encodes a copper chaperone for SOD. The AtCCS protein was localized to chloroplasts where it may supply copper to the stromal Cu/ZnSOD.
All references:	Speth et al. (2013). RACK1 scaffold proteins influence miRNA abundance in Arabidopsis. Plant J. Aug 13. Abdel-Ghany et al (2005). .AtCCS is a functional homolog of the yeast copper chaperone Ccs1/Lys7. FEBS Lett. 11:2307-12.