Chicken anti-Goat IgG (H&L), DyLight® 633 conjugated, min, cross-reactivity to human, mouse or rabbit IgG and serum proteins

AS12 2130 | Clonality: Polyclonal | Host: Chicken | Reactivity: Goat IgG (H&L)

Product Information

Immunogen

Purified goat IgG, whole molecule

Host Chicken

Clonality Polyclonal

Purity Immunogen affinity purified chicken IgG.

Format Lyophilized

Quantity 0,5 mg

Reconstitution For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Recommended dilution 1 : 20-1 : 2000 for most applications

Reactivity

Application examples

Additional information

Additional information Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative

Based on immunoelectrophoresis, this antibody reacts with: chains on goat IgG light chains on all goat immunoglobulins.

No reactivity is observed to:non-immunoglobulin goat serum proteinsIgG/serum from human, mouse or rabbit.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Background

Chicken anti-Goat IgG (H&L) - DyLight® 633 Conjugated, min. cross-reactivity to human, mouse or rabbit IgG and serum proteins is a secondary antibody conjugated to DyLight® 633, which binds to goat IgG (H&L) in immunological assays.

DyLight® 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are are affinity purified using solid phase goat IgG.

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

background:	Chicken anti-Goat IgG (H&L) - DyLight® 633 Conjugated, min. cross-reactivity to human, mouse or rabbit IgG and serum proteins is a secondary antibody conjugated to DyLight® 633, which binds to goat IgG (H&L) in immunological assays. DyLight® 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are are affinity purified using solid phase goat IgG. DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Chicken
immunogen:	Purified goat IgG, whole molecule
Purity:	Immunogen affinity purified chicken IgG.
Quantity:	0,5 mg
recommended dilution:	1 : 20-1 : 2000 for most applications
Reconstitution:	For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
storage:	Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
additional information:	Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
additional information (application):	Based on immunoelectrophoresis, this antibody reacts with: chains on goat IgG light chains on all goat immunoglobulins. No reactivity is observed to:non-immunoglobulin goat serum proteinsIgG/serum from human, mouse or rabbit. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Related products: Chicken anti-Goat IgG (H&L), DyLight® 633 conjugated, min, cross-reactivity to human, mouse or rabbit IgG and serum proteins

Chicken anti-Goat IgG (H&L), DyLight® 405 conjugated

AS16 3292 | Clonality: Polyclonal | Host: Chicken | Reactivity: Goat IgG (H&am...

268 €

Chicken anti-Goat IgG (H&L), DyLight® 633 conjugated, min, cross-reactivity to human, mouse or rabbit IgG and serum proteins

AS12 2130 | Clonality: Polyclonal | Host: Chicken | &#...

206 €

Chicken anti-Goat IgG (H&L), DyLight® 800 conjugated, min, cross-reactivity to human, mouse or rabbit IgG and serum proteins

AS12 2376 | Clonality: Polyclonal | Host: Chicken | Reactivity: Goat IgG (H&L)

220 €

Chicken anti-Goat IgG (H&L), FITC conjugated

AS10 1161 | Clonality: Polyclonal | Host: Chicken | Reactivity: Goat IgG ...

233 €