Chicken anti-Rabbit IgG (H&L), Affinity purified, Unconjugated, min. cross-reactivity to human, mouse IgG
AS10 834 | Clonality: Polyclonal | Host: Chicken | Reactivity: Rabbit IgG (H&L)
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Product Information
Immunogen
Purified Rabbit IgG
Host
Chicken
Clonality
Polyclonal
Purity
Affinity purified chicken IgY
Format
Liquid
Quantity
1 mg
Storage
Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.!!!Store non-diluted antibody at 2-8°C. For storage at -20°C dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilution prior to use and then discard.Shelf life is 1 year from date of receipt.
Tested applications
ELISA (ELISA)
Recommended dilution
The optimal working dilution should be determined by the investigator.
Reactivity
Confirmed reactivity
Rabbit IgG (H&L)
Predicted reactivity
Rabbit IgG (H&L)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.
Antibody concentration is 4.5 mg/ml.
Antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.
No reactivity is observed to non-immunoglobulin rabbit serum and human or mouse serum or IgG based on immunoelectrophoresis.
Background
Background
Chicken anti-rabbit IgG (H&L)is a secondary antibody which binds to rabbit IgG (H&L) in immunological assays.
Product citations
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