CHS | Chalcone synthase

Product no: AS12 2615

AS12 2615 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CHS, UniProt:P13114, TAIR: AT5G13930

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 µl
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunolocalization (IL), Western blot (WB)
    Recommended dilution: 1 : 20-1 : 500 (IL), 1 : 1000 (WB)
    Expected | apparent MW: 43.1 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Solanum lycopersicum
    Predicted reactivity: Cannabis sativa, Brassica sp, Gossypium hirsutum, Nicotiana tabacum, Oryza sativa, Solanum tuberosum, Petroselinum sp., Picea abies, Pisum sativum, Triticum aestivum, Zea mays, Vitis vinifera

    Species of your interest not listed? Contact us
    Not reactive in: Zostera marina
  • Application Examples
  • application example

    western blot using anti-CHS antibodies

     0.5 to 7 µg of protein from Arabidopsis thaliana Col0 leaf tissue, extracted with Agrisera PEB protein extraction buffer 1X were separated on 12 % SDS-PAGE using tank (BioRad system) to transfer to to nitrocellulose membrane during 1 hour. Blots were blocked with BSA (Sigma) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:20 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 20 seconds. For transference control the membrane was stained with Ponceau red and integrity of proteins was evaluated using 12% SDS-PAGE silver stained.

    Courtesy of Dr. Rodrigo A. Contreras, Universidad de Santiago de Chile
  • Background
  • Background: Chalcone synthase (CHS) is an essential enzyme in flavonoid biosynthesis. Required for the accumulation of purple anthocyanins in leaves and stems. Also involved in the regulation of auxin transport and the modulation of root gravitropism. Alternative names: Naringenin-chalcone synthase, Protein TRANSPARENT TESTA 4.
  • Product Citations
  • Selected references: Trojak et al. (2021) Effects of partial replacement of red by green light in the growth spectrum on photomorphogenesis and photosynthesis in tomato plants. Photosynth Res. 2021 Sep 27. doi: 10.1007/s11120-021-00879-3. Epub ahead of print. PMID: 34580802.
    Nabbie et al. (2017). Lambda Protein Affects Anthocyanin Production in Arabidopsis thaliana during Drought Stress. Journal of Agricultural Science; Vol. 9, No. 7; 2017 (immunolocalization, western blot)
  • Protocols

  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection

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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items