CHS | Chalcone synthase

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AS12 2615 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana


4 st
Item No:
AS12 2615

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product information
Background Chalcone synthase (CHS) is an essential enzyme in flavonoid biosynthesis. Required for the accumulation of purple anthocyanins in leaves and stems. Also involved in the regulation of auxin transport and the modulation of root gravitropism. Alternative names: Naringenin-chalcone synthase, Protein TRANSPARENT TESTA 4.

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CHS, UniProt:P13114, TAIR: AT5G13930

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunolocalization (IL), Western blot (WB)
Related products

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 20-1 : 500 (IL), 1 : 1000 (WB)
Expected | apparent MW

43.1 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Cannabis sativa, Brassica sp, Gossypium hirsutum, Nicotiana tabacum, Oryza sativa, Solanum tuberosum, Pisum sativum, Triticum aestivum, Zea mays, Vitis vinifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references Nabbie et al. (2017). Lambda Protein Affects Anthocyanin Production in Arabidopsis thaliana during Drought Stress. Journal of Agricultural Science; Vol. 9, No. 7; 2017 (immunolocalization, western blot)

application example

western blot using anti-CHS antibodies

 0.5 to 7 µg of protein from Arabidopsis thaliana Col0 leaf tissue, extracted with Agrisera PEB protein extraction buffer 1X were separated on 12 % SDS-PAGE using tank (BioRad system) to transfer to to nitrocellulose membrane during 1 hour. Blots were blocked with BSA (Sigma) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:20 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 20 seconds. For transference control the membrane was stained with Ponceau red and integrity of proteins was evaluated using 12% SDS-PAGE silver stained.

Courtesy of Dr. Rodrigo A. Contreras, Universidad de Santiago de Chile

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