showForm Anti-CHS | chalcone synthase antibodies

Name:
Phone:
E-mail:
Message

CHS | Chalcone synthase

AS12 2615 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

CHS | Chalcone synthase in the group Antibodies for Plant/Algal  / Hormones / Biosynthesis/regulation at Agrisera AB (Antibodies for research) (AS12 2615)

DATA SHEET IN PDF

Qty: 
278
Buy 2 items of this product for 206 €/items
Buy 3 items of this product for 190 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Datasheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CHS, UniProt:P13114, TAIR: AT5G13930

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunolocalization (IL), Western blot (WB)
Recommended dilution 1 : 20-1 : 500 (IL), 1 : 1000 (WB)
Expected | apparent MW

43.1 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Cannabis sativa, Brassica sp, Gossypium hirsutum, Nicotiana tabacum, Oryza sativa, Solanum tuberosum, Petroselinum sp., Pisum sativum, Triticum aestivum, Zea mays, Vitis vinifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

western blot using anti-CHS antibodies

 0.5 to 7 µg of protein from Arabidopsis thaliana Col0 leaf tissue, extracted with Agrisera PEB protein extraction buffer 1X were separated on 12 % SDS-PAGE using tank (BioRad system) to transfer to to nitrocellulose membrane during 1 hour. Blots were blocked with BSA (Sigma) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:20 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 20 seconds. For transference control the membrane was stained with Ponceau red and integrity of proteins was evaluated using 12% SDS-PAGE silver stained.


Courtesy of Dr. Rodrigo A. Contreras, Universidad de Santiago de Chile

Additional information

Related products

Background

Background Chalcone synthase (CHS) is an essential enzyme in flavonoid biosynthesis. Required for the accumulation of purple anthocyanins in leaves and stems. Also involved in the regulation of auxin transport and the modulation of root gravitropism. Alternative names: Naringenin-chalcone synthase, Protein TRANSPARENT TESTA 4.

Product citations

Selected references Nabbie et al. (2017). Lambda Protein Affects Anthocyanin Production in Arabidopsis thaliana during Drought Stress. Journal of Agricultural Science; Vol. 9, No. 7; 2017 (immunolocalization, western blot)

Related products: CHS | Chalcone synthase

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
From 15 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
185 €
AS15 3091 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
278 €
Buy 2 items of this product for 206 €/items
Buy 3 items of this product for 190 €/items
AS15 3092 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
278 €
Buy 2 items of this product for 206 €/items
Buy 3 items of this product for 190 €/items