slr0156 | ATP-dependent chaperone clpB
AS08 355 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Cyanobacteria

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Product Information
recombinant slr0156 protein, derived from Synechocystis PCC 6803 strain slr0156 sequence. This protein is annotated as ClpB1 in a data base but was originally named ClpB2 according to the paper of Giese and Vierling (2002).
101.4 | 100 kDa (Synechocystis)
Reactivity
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Application examples
Application example

10 μg of total protein from Synechocystis PCC 6803 (with deleted slr 1641, two left lanes) in controlled (C) and heat shocked conditions (HS) was separated on 8% PAA gel and blotted on nitrocellulose membrane. Filters were blocked (1h), incubated with 1: 3000 anti-slr0156 antibodies (2h) followed by incubation with 1: 2500 secondary anti-rabbit (1h) coupled to HRP and visualization with chemiluminescent detection reagent. Deletion of slr0156 protein is not possible, therefore there is still a band in two left lanes.
Courtesy of Courtesy of Dr. Elizabeth Vierling, University of Massachusetts, USA
Additional information
Background
ClpB2 is essential for organism and can not be complemented by a mutation in ClpB1 gene. This cytoplasmic protein is not involved in thermotolerance. Giese and Vierling (2002) Changes in oligomerization are essential for the chaperone activity of a small heat shock protein in vivo and in vitro. J Biol Chem; 277(48): 46310-8.
Product citations
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