slr0156 | ATP-dependent chaperone clpB

Product no: AS08 355

AS08 355  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Cyanobacteria

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  • Product Info
  • Immunogen:

    recombinant slr0156 protein, derived from Synechocystis PCC 6803 strain slr0156 sequence. This protein is annotated as ClpB1 in a data base but was originally named ClpB2 according to the paper of Giese and Vierling (2002).

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 100 l
    Reconstitution: For reconstitution add 100 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 3000 (WB)
    Expected | apparent MW:

    101.4 | 100 kDa (Synechocystis)

  • Reactivity
  • Confirmed reactivity: Synechocystis PCC 6803
    Predicted reactivity: Cyanobacteria
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

     

    western blot detection of slr0156 protein in cyanobacteria  

    10 μg of total protein from Synechocystis PCC 6803 (with deleted slr 1641, two left lanes) in controlled (C) and heat shocked conditions (HS) was separated on 8% PAA gel and blotted on nitrocellulose membrane. Filters were blocked (1h), incubated with 1: 3000 anti-slr0156 antibodies (2h) followed by incubation with 1: 2500 secondary anti-rabbit (1h) coupled to HRP and visualization with chemiluminescent detection reagent.  Deletion of slr0156 protein is not possible, therefore there is still a band in two left lanes.

    Courtesy of Courtesy of Dr. Elizabeth Vierling, University of Massachusetts, USA
  • Background
  • Background:

    ClpB2 is essential for organism and can not be complemented by a mutation in ClpB1 gene. This cytoplasmic protein is not involved in thermotolerance. Giese and Vierling (2002) Changes in oligomerization are essential for the chaperone activity of a small heat shock protein in vivo and in vitro. J Biol Chem; 277(48): 46310-8.

  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts


    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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