slr1641 | ATP-dependent chaperone clpB
AS08 344 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Cyanobacteria
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Product Information
Immunogen
recombinant clpB1 protein, derived from Synechocystis PCC 6803 strain slr1641 sequence; protein has an internal translation site. The nomenclature used is reverse of what is mentioned in the cyanobase.
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
100 ĩl
Reconstitution
For reconstitution add 100 ĩl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 3000 (WB)
Expected | apparent MW
98.1 | 85.4 and 105 | 95 kDa for Synechocystis
Reactivity
Confirmed reactivity
Synechocystis PCC 6803, Solanum lycopersicum
Predicted reactivity
Cyanobacteria, Francisella sp.
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
Chlamydomonas reinhardtii
Application examples
Application examples
Application example
10 μg of total protein from Synechocystis PCC 6803 wild type (+ClpB1) and slr1641 deletion mutant, control (C) and heat shocked samples (HS) was separated on 8% PAA gel and blotted on nitrocellulose membrane . Filters were blocked (1h), incubated with 1: 3000 anti-ClpB1 antibodies (2h) followed by incubation with 1: 2500 secondary anti-rabbit (1h) coupled to HRP and visualization with chemiluminescent detection reagent.
Courtesy of Dr. Elizabeth Vierling, University of Massachusetts, USA
10 μg of total protein from Synechocystis PCC 6803 wild type (+ClpB1) and slr1641 deletion mutant, control (C) and heat shocked samples (HS) was separated on 8% PAA gel and blotted on nitrocellulose membrane . Filters were blocked (1h), incubated with 1: 3000 anti-ClpB1 antibodies (2h) followed by incubation with 1: 2500 secondary anti-rabbit (1h) coupled to HRP and visualization with chemiluminescent detection reagent.
Courtesy of Dr. Elizabeth Vierling, University of Massachusetts, USA
Additional information
Background
Background
ClpB protein is essential for resistance to high temperature stress. It functions to dissolve inactive protein aggregates that accumulate at high temperatures. Giese and Vierling (2002) Changes in oligomerization are essential for the chaperone activity of a small heat shock protein in vivo and in vitro. J Biol Chem; 277(48): 46310-8.
Product citations
Selected references
Gonzalez-Esquer and Vermaas (2013). ClpB1 overproduction in Synechocystis sp. strain PCC 6803 increases tolerance to rapid heat shock. Appl Environ Microbiol. 2013 Oct;79(20):6220-7. doi: 10.1128/AEM.01661-13. Epub 2013 Aug 2.
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