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CRY2 | Cryptochrome 2
AS18 4221 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
- Product Info
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Immunogen: Part of Arabidopsis thaliana CRY2 protein sequence, UniProt:Q96524, TAIR: At1g04400
Antigen used to elicit CR2 antibodies is not conserved in CRY1 protein.Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl, of sterile water. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 69.5 | 70 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Camelina sativa, Capsella rubella
Species of your interest not listed? Contact usNot reactive in: Poplar sp. - Application Examples
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Samples:
50 µg of Arabidopsis thaliana whole seedlings (7 days old)
1 - cry1-304 (overexpressing line)
2 - cry2-1 (deletion mutant of both isoforms)
3 - cry1-304 cry2-1 (substitution mutant)
Mark: MW markers: PageRuler™ Plus Prestained Protein Ladder from Thermo Scientific™ (26619)8 µl/well of total protein extracted freshly from 50 ug of Arabidopsis thaliana seedlings (7-day WL grown) with 8M Urea, 0.35M Tris-Cl (pH 7.5), 1× protease inhibitor cocktail. and denatured with 6x SDS buffer at 95°C for 10 min. Samples were separated o 4-12 % gradient SDS-PAGE (Invitrogen) and transferred 7 min to nitrocellulose (pore size of 0.2 µm), using transfer device (iBlot2, Thermofischer). Blot was blocked with 2 % milk 1h/RTwith agitation. Blot was incubated in the primary antibody at a dilution of 1: 1,000 for ON/4°C without agitation. The antibody solution was decanted and the blot was washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (goat anti-rabbit HRP conjugated AS09 602) diluted to 1:25 000 in for 3h/RT with agitation. The blot was washed as above and developed with AgriseraECLSuperBright AS16 ECL-S-100, using Summit QCP. Exposure time was 5 seconds.
Courtesy of Dr. Sanghwa Lee, Salk Institute for Biological Studies, USA
- Background
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Background: CRY2 (Cryptochrome 2) is a photoreceptor that mediates primarily blue light inhibition of hypocotyl elongation and photoperiodic control of floral initiation, and regulates other light responses: circadian rhythms, tropic growth, stomata opening, guard cell development, root development, bacterial and viral pathogen responses, abiotic stress responses, cell cycles, programmed cell death, apical dominance, fruit and ovule development, seed dormancy, and magnetoreception. (PubMed:21841916).
Alternative protein names: Blue light photoreceptor1, Protein PHR homolog 11 AtPHH1, Protein SUPPRESSOR OF elf3 20
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection - Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
10 % until end of 2023. Use discount code: Conj10
This product can be purchased in 3 different volumes:
AS16 ECL-S-10, 10 ml. Trial size limited to one per customer
AS16 ECL-S-100, 100 ml
AS16 ECL-S-1L, 1L
Choose the appropriate volume in the drop down menu to the right
AS16 3933 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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