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CRY2 | Cryptochrome 2

AS18 4221   | Clonality: Polyclonal |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

CRY2 | Cryptochrome 2 in the group Antibodies Plant/Algal  / Developmental Biology / Photomorphogenesis at Agrisera AB (Antibodies for research) (AS18 4221)
CRY2 | Cryptochrome 2



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Product Information

Immunogen Part of Arabidopsis thaliana CRY2 protein sequence, UniProt:Q96524, TAIR: At1g04400
Antigen used to elicit CR2 antibodies is not conserved in CRY1 protein.
Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 69.5 | 70 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Camelina sativa, Capsella rubella
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Western blot using anti-CRY2 antiodies

Samples:
50 µg of Arabidopsis thaliana whole seedlings (7 days old)
1 - cry1-304 (overexpressing line)
2 - cry2-1 (deletion mutant of both isoforms)
3 - cry1-304 cry2-1 (substitution mutant)

Mark: MW markers: PageRuler™ Plus Prestained Protein Ladder from Thermo Scientific™ (26619)

8 µl/well of total protein extracted freshly from 50 ug of Arabidopsis thaliana seedlings (7-day WL grown) with 8M Urea, 0.35M Tris-Cl (pH 7.5), 1× protease inhibitor cocktail. and denatured with 6x SDS buffer at 95°C for 10 min. Samples were separated o 4-12 % gradient SDS-PAGE (Invitrogen) and transferred 7 min to nitrocellulose (pore size of 0.2 µm), using transfer device (iBlot2, Thermofischer). Blot was blocked with 2 % milk 1h/RTwith agitation. Blot was incubated in the primary antibody at a dilution of 1: 1,000 for ON/4°C without agitation. The antibody solution was decanted and the blot was washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (goat anti-rabbit HRP conjugated AS09 602) diluted to 1:25 000 in for 3h/RT with agitation. The blot was washed as above and developed with AgriseraECLSuperBright AS16 ECL-S-100, using Summit QCP. Exposure time was 5 seconds.

Courtesy of Dr. Sanghwa Lee, Salk Institute for Biological Studies, USA

Additional information

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Background

Background CRY2 (Cryptochrome 2) is a photoreceptor that mediates primarily blue light inhibition of hypocotyl elongation and photoperiodic control of floral initiation, and regulates other light responses: circadian rhythms, tropic growth, stomata opening, guard cell development, root development, bacterial and viral pathogen responses, abiotic stress responses, cell cycles, programmed cell death, apical dominance, fruit and ovule development, seed dormancy, and magnetoreception. (PubMed:21841916).

Alternative protein names: Blue light photoreceptor1, Protein PHR homolog 11 AtPHH1, Protein SUPPRESSOR OF elf3 20

Product citations

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