CRY1 | Cryptochrome 1
AS16 3933 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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15 µg of total protein from Arabidopsis thaliana etiolated/greening seedling extracted with exact buffer (0.125 M Tris, 1% SDS, 10% Glycerol, 0.05 M Sodium metabisulfite) and denatured at 70°C for 5 min. Samples and Prestained Protein were separated on 10% SDS-PAGE(1.5 mm, 10-well) and blotted 50 min to PVDF using semi-dry. Blots were blocked with 5% nonfat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for overnight at 4°C with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:5000 in 1% nonfat dry milk for 4h at RT with agitation. The blot was washed as above and developed for 2 min with ECL reagent. Exposure time was 10 min.
Courtesy of Duorong Xu, LMU, Munich, Germany
Alternative names: Blue light photoreceptor1, Protein BLUE LIGHT UNINHIBITED 1, Protein ELONGATED HYPOCOTYL 4, Protein OUT OF PHASE 2, OOP2.
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