CRY1 | Cryptochrome 1

Product no: AS16 3933

AS16 3933 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
319
Add to cart
Delivery:  3-6 business days
  • Data sheet
  • Product Info
  • Immunogen: KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana CRY1, UniProt:Q43125, TAIR:AT4G08920

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 76,6 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Nicotiana tabacum
    Predicted reactivity: Cajanus cajan, Capsella orientalis, Corchorus capsularis, Fragaria ananassa, Fragaria vesca, Gossypium hirsutum, Medicago truncatula, Nelumbo nucifera, Populus tremula, Zostera marina
    Species of your interest not listed? Contact us
    Not reactive in: diatoms
  • Application Examples
  • Application example


    western blot using anti-CRY1 antibodies
    15 µg of total protein from Arabidopsis thaliana etiolated/greening seedling extracted with exact buffer (0.125 M Tris, 1% SDS, 10% Glycerol, 0.05 M Sodium metabisulfite) and denatured at 70°C for 5 min. Samples and Prestained Protein were separated on 10% SDS-PAGE(1.5 mm, 10-well) and blotted 50 min to PVDF using semi-dry. Blots were blocked with 5% nonfat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for overnight at 4°C with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:5000 in 1% nonfat dry milk for 4h at RT with agitation. The blot was washed as above and developed for 2 min with ECL reagent. Exposure time was 10 min.

    Courtesy of Duorong Xu, LMU, Munich, Germany
  • Background
  • Background: CRY1 (Cryptochrome 1) is a flavin-type blue-light photoreceptor with ATP binding and autophosphorylation activity and functions in perception of blue / green ratio of light. It regulates other light responses, including circadian rhythms, tropic growth, stomata opening, guard cell development, root development, bacterial and viral pathogen responses, abiotic stress responses, cell cycles, programmed cell death, apical dominance, fruit and ovule development, seed dormancy, and magnetoreception.
    Alternative names: Blue light photoreceptor1, Protein BLUE LIGHT UNINHIBITED 1, Protein ELONGATED HYPOCOTYL 4, Protein OUT OF PHASE 2, OOP2.
  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (1)
    Andreas | 2021-07-22
    0 | 0
    The antibody worked well on denatured Arabidopsis whole cell protein extracts at a dilution of 1:2000 (overnight 4C in 1% milk TBS).

Accessories

AS06 198 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Oryza sativa

319 €
Add to cart
Info
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

213 €
Add to cart
Info
img missing
AS16 ECL-S | extreme low femtogram detection

This product can be purchased in 3 different volumes:

AS16 ECL-S-10, 10 ml. Trial size limited to one per customer

AS16 ECL-S-100, 100 ml

AS16 ECL-S-1L, 1L

Choose the appropriate volume in the drop down menu to the right

From 17 €

AS16 3954 | Clonality: Polyclonal | Host: Goat | Reactivity: Arabidopsis thaliana

319 €
Add to cart
Info
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
Close