DCL2 | Dicer-like protein 2

AS15 3100 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

DCL2 | Dicer-like protein 2 in the group Plant/Algal Antibodies / DNA/RNA/Cell Cycle / Transcription regulation at Agrisera AB (Antibodies for research) (AS15 3100)


355 €

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product information

Endoribonuclease Dicer homolog 2 is a protein which processes secondary siNAs, which is an important part in the transitive silencing of transgenes. Alternative names: Dicer-like protein 2

Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana DCL2 sequence, Uniprot: Q3EBC8, TAIR: AT3G03300
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 200 µg
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS12 2102 | anti-DCL1 | Dicer-like protein 1, rabbit antibodies
AS12 2103
| anti-DCL3 | Dicer-like protein 3, rabbit antibodies
AS15 3105 | anti-DCL4 | Dicer-like protein 4, rabbit antibodies

collection of antibodies to proteins involved in microRNA metabolism

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 5000-1 : 10 000 (WB)
Expected | apparent MW

156.9 | 157 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity
Not reactive in

Nicotiana tabacum, Zea mays

Additional information
Selected references

to be added when available, antibody released in January 2016.

application example

western blot using anti-DCL2 antibodies

50 µg of total protein from Arabidopsis thaliana whole vegetative rosette, DCL2 overexpression line (a), wild type Col-0 (b), dcl2-1 intron insertion (c),  extracted with extraction buffer (50 mM Tris pH7.5; 150 mM NaCl; 1 mM EDTA; 10 % v/v Glycerin; 1 mM DTT, 1x Complete Protease Inhibitor Cocktail, Roche) and denatured with laemmli buffer at 95°C/5 min. were separated on 7.5 % SDS-PAGE and blotted 1.5 h to PVDF using tank transfer. Blots were blocked with blocking buffer (5% milk powder; 1x TBS; 0.1% Tween-20) overnight at 4°C with agitation. Blot was incubated in the primary antibody at a dilution of 1:1000 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly and then washed tree times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:20 000 in blocking buffer for 1h at RT with agitation. The blot was washed as above and developed for 5 min with Amersham ECL Prime and expose to Amersham Hyperfilms ECL for 120 seconds.

Courtesy of Dr. Dr. Pablo Manavella, Instituto de Agrobiotecnología del Litoral (IAL), Argentina

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