DMPO | DMPO nitrone adduct (clone N1664A)

Name: DMPO | DMPO nitrone adduct (clone N1664A)
Brand: IKP
SKU: AS11 1632
Price: 439 EUR
Availability: InStock

AS11 1632 | Clonality: monoclonal | Host:Mouse | Reactivity:DMPO

DMPO | DMPO nitrone adduct (clone N1664A) in the group Antibodies Plant/Algal / Environmental Stress / Plant Oxidative Stress at Agrisera AB (Antibodies for research) (AS11 1632)

DMPO | DMPO nitrone adduct (clone N1664A)

Product Information

Immunogen 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to ovalbumin

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG1

Purity Total IgG. Protein G purified.

Format Liquid

Quantity 100 µl

Storage Store at -20°C for one year, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immunocytochemistry (ICC), Immuno-spin trapping (IT), Immunoprecipitation (IP), Western blot (WB)

Recommended dilution 1-10 µg/ml (ELISA), 10 µg/ml (ICC), 25 µg (IP), 1-10 µg/ml (WB)

Expected | apparent MW

90 kDa

Reactivity

Confirmed reactivity DMPO (species independent)

Predicted reactivity DMPO (species independent)

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Antibody is purified on Protein G and present in TBS pH 7.4 with 0.05 % sodium azide as preservative and 50 % glycerol.

DMPO antibody in dilution of 1: 1000 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colormetric immunoblot analysis using goat anti-mouse IgG HRP conjugated secondary antibody.

Important note for immunoprecipitation: do not link antibody to matrix prior to use as this causes large loss of reactivity to DMPO adduct

Background

DMPO (5,5-dimethly-1-proline N-oxide) is a compound used in spin trapping. It is a method employed in chemistry as an analytical technique for detection and identification of short-lived free radicals. DMPO adducts can be generated with protein and DNA radicals.

Product citations

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immunogen:	5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to ovalbumin
Sub class:	IgG1
Host:	Mouse
Clonality:	Monoclonal
Purity:	Total IgG. Protein G purified.
Format:	Liquid
Quantity:	100 µl
storage:	Store at -20°C for one year, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Immunocytochemistry (ICC), Immuno-spin trapping (IT), Immunoprecipitation (IP), Western blot (WB)
recommended dilution:	1-10 µg/ml (ELISA), 10 µg/ml (ICC), 25 µg (IP), 1-10 µg/ml (WB)
calculated \| apparent molecular mass [kDa]:	90 kDa
Confirmed reactivity:	DMPO (species independent)
predicted reactivity:	DMPO (species independent)
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	Antibody is purified on Protein G and present in TBS pH 7.4 with 0.05 % sodium azide as preservative and 50 % glycerol. DMPO antibody in dilution of 1: 1000 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colormetric immunoblot analysis using goat anti-mouse IgG HRP conjugated secondary antibody.
additional information (application):	Important note for immunoprecipitation: do not link antibody to matrix prior to use as this causes large loss of reactivity to DMPO adduct
background:	DMPO (5,5-dimethly-1-proline N-oxide) is a compound used in spin trapping. It is a method employed in chemistry as an analytical technique for detection and identification of short-lived free radicals. DMPO adducts can be generated with protein and DNA radicals.