IKP

DMPO | DMPO nitrone adduct (clone N1664A)

Product no: AS11 1632

AS11 1632   | Clonality: monoclonal  |  Host:Mouse |  Reactivity:DMPO

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  • Product Info
  • Immunogen: 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to ovalbumin
    Sub class: IgG1
    Host: Mouse
    Clonality: Monoclonal
    Purity: Total IgG. Protein G purified.
    Format: Liquid
    Quantity: 100 l
    Storage: Store at -20C for one year, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Immunocytochemistry (ICC), Immuno-spin trapping (IT), Immunoprecipitation (IP), Western blot (WB)
    Recommended dilution: 1-10 µg/ml (ELISA), 10 µg/ml (ICC), 25 µg (IP), 1-10 µg/ml (WB)
    Expected | apparent MW:

    90 kDa

  • Reactivity
  • Confirmed reactivity: DMPO (species independent)
    Predicted reactivity: DMPO (species independent)
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information:

    Antibody is purified on Protein G and present in TBS pH 7.4 with 0.05 % sodium azide as preservative and 50 % glycerol.

    DMPO antibody in dilution of 1: 1000 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colormetric immunoblot analysis using goat anti-mouse IgG HRP conjugated secondary antibody.

    Additional information (application): Important note for immunoprecipitation: do not link antibody to matrix prior to use as this causes large loss of reactivity to DMPO adduct
  • Background
  • Background:

    DMPO (5,5-dimethly-1-proline N-oxide) is a compound used in spin trapping. It is a method employed in chemistry as an analytical technique for detection and identification of short-lived free radicals. DMPO adducts can be generated with protein and DNA radicals.

  • Product Citations
  • Selected references: Chatterjee et al. (2009). Immuno-spin trapping of a post-translational carboxypeptidase B1 radical formed by a dual role of xanthine oxidase and endothelial nitric oxide synthase in acute septic mice. Free Radic. Med. and Biol. 46:454-461.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection

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