showForm Donkey anti-Goat IgG (H&L), DyLightŪ488 Conjugated

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Donkey anti-Goat IgG (H&L), DyLightŪ 488 conjugated

AS10 1116 | Clonality: Polyclonal | Host: Donkey | Reactivity: Goat IgG (H&L)

Donkey anti-Goat IgG (H&L), DyLightŪ 488 conjugated in the group Secondary Antibodies / Anti-Goat / Fluorescent / DyLightŪ / DyLightŪ 488 at Agrisera AB (Antibodies for research) (AS10 1116)

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Product Information

Immunogen

Purified Goat IgG, whole molecule

Host Donkey
Clonality Polyclonal
Purity Affinity purified donkey IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution 1 : 20-1 : 2000 for most applications

Reactivity

Predicted reactivity
Not reactive in

Application examples

Application examples

Additional information

Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

Based on immunoelectrophoresis, this antibody reacts with:, heavy chains on goat IgG, light chains on all goat immunoglobulins

No reactivity is observed to, non-immunoglobulin goat serum proteins

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Related products

Background

Background

Donkey anti-Goat IgG (H&L), DyLight®488 Conjugated is a secondary antibody conjugated to DyLight® 488, which binds to Goat IgG (H&L) in immunological assays.

DyLight® 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Goat IgG (H&L)

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

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