showForm Donkey anti-Mouse IgG (H&L), DyLightŪ488 Conjugated, min. cross

Name:
Phone:
E-mail:
Message

Donkey anti-Mouse IgG (H&L), DyLightŪ 488 conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG

AS10 1201 | Clonality: Polyclonal | Host: Donkey | Reactivity: Mouse IgG (H&L)

Donkey anti-Mouse IgG (H&L), DyLightŪ 488 conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human,  rabbit, rat or sheep IgG in the group Secondary Antibodies / Anti-Mouse / Fluorescent / DyLightŪ / DyLightŪ 488 at Agrisera AB (Antibodies for research) (AS10 1201)

DATA SHEET IN PDF

Qty: 
227
How to cite this product:
Product name, number (Agrisera, Sweden)

Datasheet Product citations Protocols Add review

Product Information

Immunogen

Purified Mouse IgG, whole molecule

Host Donkey
Clonality Polyclonal
Purity Affinity purified donkey IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution 1 : 20-1 : 2000 for most applications

Reactivity

Predicted reactivity
Not reactive in

Application examples

Application examples

Additional information

Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG, light chains on all mouse immunoglobulins

No reactivity is observed to: non-immunoglobulin mouse serum proteins, IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep

Related products

Background

Background

Donkey anti-Mouse IgG (H&L), DyLight®488 Conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG is a secondary antibody conjugated to DyLight® 488, which binds to Mouse IgG (H&L) in immunological assays.

DyLight® 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Mouse IgG (H&L)

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

Selected references Maurya et al. (2017). Hedgehog signaling regulates ciliary localization of mouse odorant receptors. Proc Natl Acad Sci U S A. 2017 Oct 31;114(44):E9386-E9394. doi: 10.1073/pnas.1708321114.