Donkey anti-Rabbit IgG (H&L), HRP conjugated, min, cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG
AS10 1008 | Clonality: Polyclonal | Host: Donkey | Reactivity: Rabbit IgG (H&L)
- Product Info
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Host: Donkey Clonality: Polyclonal Purity: Immunogen affinity purified IgG. Format: Lyophilized Quantity: 1 mg Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. Recommended dilution: The optimal working dilution should be determined by the investigator - Additional Information
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Additional information: This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.
Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
- Background
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Background: Donkey anti-rabbit IgG (H&L) is a secondary antibody, HRP conjugated, which binds to all rabbit IgGs in immunological assays. Antibody is specially adsorbed against bovine,chicken,goat,guinea pig,hamster,horse,human,mouse,rat,sheep IgG. Antibodies are affinity purified using solid phase rabbit IgG. - Product Citations
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Selected references: Petersen and Andersen (2014). Simultaneous isolation of mRNA and native protein from minute samples of cells. Biotechniques. 2014 May 1;56(5):229-37. doi: 10.2144/000114165. eCollection 2014.
- Protocols
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Accessories
AS10 1269 | Clonality: Polyclonal | Host:Donkey | Reactivity: Rabbit IgG (H&L)
AS10 1299 | Clonality: Polyclonal | Host:Donkey | Reactivity: Rabbit IgG (H&L)
AS10 842 | Clonality: Polyclonal | Host: Donkey | Reactivity: Rabbit IgG (heavy and light chains - H&L)