Donkey anti-Sheep IgG (H&L), DyLight® 800 conjugated, min, cross-reactivity to human, mouse, or rabbit IgG

AS12 2479 | Clonality: Polyclonal | Host: Donkey | Reactivity: Sheep IgG (H&L)

Product Information

Immunogen

Purified Sheep IgG, whole molecule

Host Donkey

Clonality Polyclonal

Purity Immunogen affinity purified donkey IgG.

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Tested applications immunofluorescence (IF), IImmunohistochemisty (IHC), Western blot (WB)

Recommended dilution 1 : 20-1 : 2000 for most applications

Reactivity

Not reactive in

Application examples

Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on sheep IgG, light chains on all sheep immunoglobulins

No reactivity is observed to: non-immunoglobulin sheep serum proteins, IgG from human, mouse or rabbit.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Background

Donkey anti-Sheep IgG (H&L), DyLight®800 Conjugated, min. cross-reactivity to human,mouse, or rabbit IgG is a secondary antibody conjugated to DyLight® 800, which binds to Sheep IgG (H&L) in immunological assays.

DyLight® 800 has Amax = 777 nm, Emax = 794 nm. Antibodies are purified using solid phase Sheep IgG (H&L)

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

background:	Donkey anti-Sheep IgG (H&L), DyLight®800 Conjugated, min. cross-reactivity to human,mouse, or rabbit IgG is a secondary antibody conjugated to DyLight® 800, which binds to Sheep IgG (H&L) in immunological assays. DyLight® 800 has Amax = 777 nm, Emax = 794 nm. Antibodies are purified using solid phase Sheep IgG (H&L) DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
not reactive in:
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Donkey
immunogen:	Purified Sheep IgG, whole molecule
Purity:	Immunogen affinity purified donkey IgG.
Quantity:	1 mg
recommended dilution:	1 : 20-1 : 2000 for most applications
Reconstitution:	For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
storage:	Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
tested applications:	immunofluorescence (IF), IImmunohistochemisty (IHC), Western blot (WB)
Picture (footer):
additional information:	Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative. Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on sheep IgG, light chains on all sheep immunoglobulins No reactivity is observed to: non-immunoglobulin sheep serum proteins, IgG from human, mouse or rabbit. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
additional information (application):

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