showForm Donkey anti-sheep IgG (H&L), FITC conjugated, adsorbed

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Donkey anti-Sheep IgG (H&L), FITC conjugated, min. cross-reactivity to human, rabbit IgG

AS10 1148   | Clonality: Polyclonal  |  Host: Donkey  |  Reactivity:Sheep IgG (H&L)

Donkey anti-Sheep IgG (H&L), FITC conjugated, min. cross-reactivity to human, rabbit IgG  in the group Secondary Antibodies / Anti-Sheep / Fluorescent / FITC, TRITC at Agrisera AB (Antibodies for research) (AS10 1148)

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Product Information

Host Donkey
Clonality Polyclonal
Purity Affinity purified IgG
Format Liquid
Quantity 2 mg
Storage Store at 2-8°C. Shelf life of this product is one year from date of receipt.
Recommended dilution 1 : 20-1 : 2000 (IHC), 1 : 50-1 : 5000 (ICC) (Flow cyt)

Reactivity

Application examples

Additional information

Additional information

This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins and human,rabbit IgG based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, ,1% (w/v) BSA, Protease/IgG free, 0.05 % (w/v) sodium azide as preservative.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

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Background

Background Donkey anti-sheep IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to sheep IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against human,rabbit IgG and affinity purified using solid phase sheep IgG.

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