Donkey anti-Sheep IgG (H&L), FITC conjugated, min, cross-reactivity to human, rabbit IgG

AS10 1148 | Clonality: Polyclonal | Host: Donkey | Reactivity: Sheep IgG (H&L)

Product Information

Host Donkey

Clonality Polyclonal

Purity Immunogen affinity purified IgG.

Format Liquid

Quantity 2 mg

Storage Store at 2-8°C. Shelf life of this product is one year from date of receipt.

Recommended dilution 1 : 20-1 : 2000 (IHC), 1 : 50-1 : 5000 (ICC) (Flow cyt)

Reactivity

Application examples

Additional information

This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins and human,rabbit IgG based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, ,1% (w/v) BSA, Protease/IgG free, 0.05 % (w/v) sodium azide as preservative.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Background

Background Donkey anti-sheep IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to sheep IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against human,rabbit IgG and affinity purified using solid phase sheep IgG.

Product citations

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Clonality:	Polyclonal
Format:	Liquid
Host:	Donkey
Purity:	Immunogen affinity purified IgG.
Quantity:	2 mg
recommended dilution:	1 : 20-1 : 2000 (IHC), 1 : 50-1 : 5000 (ICC) (Flow cyt)
storage:	Store at 2-8°C. Shelf life of this product is one year from date of receipt.
background:	Donkey anti-sheep IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to sheep IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against human,rabbit IgG and affinity purified using solid phase sheep IgG.
additional information:	This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins and human,rabbit IgG based on immunoelectrophoresis. Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, ,1% (w/v) BSA, Protease/IgG free, 0.05 % (w/v) sodium azide as preservative. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.