Donkey anti-Sheep IgG (H&L), TRITC conjugated, min, cross-reactivity to human, mouse,rabbit IgG

Product no: AS10 1134

AS10 1134 | Clonality: Polyclonal  |  Host: Donkey   |  Reactivity: Sheep IgG (H&L)

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  • Product Info
  • Host: Donkey
    Clonality: Polyclonal
    Purity: Immunogen affinity purified IgG.
    Format: Liquid
    Quantity: 1 mg
    Storage: Store at 2-8°C. Shelf life of this product is one year from date of receipt.
    Recommended dilution: The optimal working dilution should be determined by the investigator,
  • Additional Information
  • Additional information:

    This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.

    Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins and human,mouse,rabbit IgG based on immunoelectrophoresis.

    Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, ,1% (w/v) BSA, Protease/IgG free, 0.05 % (w/v) sodium azide as preservative.

    BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

  • Background
  • Background: Donkey anti-sheep IgG (H&L), Rhodamine (TRITC-tetramethyl rhodamine-5-isothiocyanate) conjugated, is a secondary antibody which binds to sheep IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are adsorbed against and human,mouse,rabbit IgG  and affinity purified using solid phase sheep IgG.
  • Protocols
  • Antibody protocols
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