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Donkey anti-Sheep IgG (H&L), HRP conjugated, min. cross-reactivity to human, rabbit IgG

AS10 1160   | Clonality: Polyclonal  |  Host: Donkey   |  Reactivity: Sheep IgG (H&L)

Donkey anti-Sheep IgG (H&L), HRP conjugated, min. cross-reactivity to human, rabbit IgG in the group Secondary Antibodies / Anti-Sheep / HRP (horse radish peroxidase) at Agrisera AB (Antibodies for research) (AS10 1160)

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Product Information

Host Donkey
Clonality Polyclonal
Purity Affinity purified IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution 1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)

Reactivity

Application examples

Additional information

Additional information

This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins based on immunoelectrophoresis. Minimum cross-reactivity is observed with IgG from human or rabbit based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Related products

Background

Background Donkey anti-sheep IgG (H&L),HRP conjugated, is a secondary antibody which binds to sheep IgGs in immunological assays. Antibodies are adsorbed against  human,rabbit IgG and affinity purified using solid phase sheep IgG.

Product citations

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