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Donkey anti-Sheep IgG (H&L), HRP conjugated
AS09 604 | Clonality: Polyclonal | Host: Donkey | Reactivity: Sheep IgG (H&L)
- Product Info
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Immunogen: Purified sheep IgG, whole molecule
Host: Donkey Clonality: Polyclonal Purity: Immunogen affinity purified donkey IgG. Format: Lyophilized Quantity: 1 mg Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily Storage: Store lyophilized material at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. Tested applications: ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: 1 : 200 -1 : 5 000 (ELISA), 1 : 200-1 : 5 000 (WB) 1 : 500 -1 : 5000 (IHC) - Reactivity
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Confirmed reactivity: Sheep IgG heavy chains and light chains on all sheep immunoglobulins Not reactive in: Non-immunoglobulin sheep serum proteins based in immunoelectrophoresis - Additional Information
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Additional information: Antibody concentration is 1 mg/ml and it has been purified by antigen-specific chromatography
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free
0.1 % (v/v) of ProClin 150 is used as preservative.
For blocking: BSA and nonfat milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
- Background
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Background: Donkey anti-sheep IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all sheep immunoglobulins in immunological assays.
- Protocols
- Agrisera Western Blot protocol and video tutorials
- Reviews:
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