Donkey anti-Sheep IgG (H&L), HRP conjugated

AS09 604 | Clonality: Polyclonal | Host: Donkey | Reactivity: Sheep IgG (H&L)

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Product Information

Immunogen

Purified sheep IgG, whole molecule

Host Donkey

Clonality Polyclonal

Purity Affinity purified using solid phase sheep IgG

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily.

Storage Store lyophilized material at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Tested applications ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 200 -1 : 5 000 (ELISA), 1 : 200-1 : 5 000 (WB) 1 : 500 -1 : 5000 (IHC)

Reactivity

Confirmed reactivity Sheep IgG heavy chains and light chains on all sheep immunoglobulins

Not reactive in non-immunoglobulin sheep serum proteins based in immunoelectrophoresis

Application examples

Additional information

Antibody concentration is 1 mg/ml and it has been purified by antigen-specific chromatography

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of ProClin 150 is used as preservative.

For blocking: BSA and nonfat milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Related products

AS09 611 | Donkey anti-sheep IgG (H&L) biotin conjugated

Collection of secondary antibodies

Background

Donkey anti-sheep IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all sheep immunoglobulins in immunological assays.