Donkey anti-Sheep IgG (H&L), HRP conjugated

AS09 604 | Clonality: Polyclonal | Host: Donkey | Reactivity: Sheep IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

Product Information

Immunogen

Purified sheep IgG, whole molecule

Host Donkey

Clonality Polyclonal

Purity Immunogen affinity purified donkey IgG.

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Tested applications ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 200 -1 : 5 000 (ELISA), 1 : 200-1 : 5 000 (WB) 1 : 500 -1 : 5000 (IHC)

Reactivity

Confirmed reactivity Sheep IgG heavy chains and light chains on all sheep immunoglobulins

Not reactive in non-immunoglobulin sheep serum proteins based in immunoelectrophoresis

Application examples

Additional information

Antibody concentration is 1 mg/ml and it has been purified by antigen-specific chromatography

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of ProClin 150 is used as preservative.

For blocking: BSA and nonfat milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Background

Donkey anti-sheep IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all sheep immunoglobulins in immunological assays.

Product citations

Related products: Donkey anti-Sheep IgG (H&L), HRP conjugated

Agrisera ECL kit (Bright/SuperBright)

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...

From 26 €

Info

Add to cart

AgriseraECL Bright

AS16 ECL-N | low pico to mid femtogram detection

This product can be purchased in 3 ...

From 12 €

Info

Add to cart

AgriseraECL SuperBright

AS16 ECL-S | extreme low femtogram detection

This product can be purchased in 3 different volum...

From 16 €

Info

Add to cart

Donkey anti-Sheep IgG (H&L), Biotin conjugated

AS09 611 | Clonality: Polyclonal | Host: Donkey | Reactivity: Sheep IgG (H&L...

206 €

background:	Donkey anti-sheep IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all sheep immunoglobulins in immunological assays.
Confirmed reactivity:	Sheep IgG heavy chains and light chains on all sheep immunoglobulins
not reactive in:	non-immunoglobulin sheep serum proteins based in immunoelectrophoresis
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Donkey
immunogen:	Purified sheep IgG, whole molecule
Purity:	Immunogen affinity purified donkey IgG.
Quantity:	1 mg
recommended dilution:	1 : 200 -1 : 5 000 (ELISA), 1 : 200-1 : 5 000 (WB) 1 : 500 -1 : 5000 (IHC)
Reconstitution:	For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
storage:	Store lyophilized material at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
tested applications:	ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
additional information:	Antibody concentration is 1 mg/ml and it has been purified by antigen-specific chromatography HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of ProClin 150 is used as preservative. For blocking: BSA and nonfat milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.