Donkey anti-Sheep IgG (H&L), HRP conjugated

Product no: AS09 604

AS09 604 | Clonality: Polyclonal  |  Host: Donkey  | Reactivity: Sheep IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

206
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Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    Purified sheep IgG, whole molecule

    Host: Donkey
    Clonality: Polyclonal
    Purity: Immunogen affinity purified donkey IgG.
    Format: Lyophilized
    Quantity: 1 mg
    Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
    Storage: Store lyophilized material at 2-8C. For storage at -20C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
    Tested applications: ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: 1 : 200 -1 : 5 000 (ELISA), 1 : 200-1 : 5 000 (WB) 1 : 500 -1 : 5000 (IHC)
  • Reactivity
  • Confirmed reactivity: Sheep IgG heavy chains and light chains on all sheep immunoglobulins
    Not reactive in: non-immunoglobulin sheep serum proteins based in immunoelectrophoresis
  • Additional Information
  • Additional information:

    Antibody concentration is 1 mg/ml and it has been purified by antigen-specific chromatography

    HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

    0.1 % (v/v) of ProClin 150 is used as preservative.

    For blocking: BSA and nonfat milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

  • Background
  • Background:

    Donkey anti-sheep IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all sheep immunoglobulins in immunological assays.

  • Protocols
  • Agrisera Western Blot protocol and video tutorials
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Accessories

AS09 611 | Clonality: Polyclonal  |  Host: Donkey  | Reactivity: Sheep IgG (H&L)

206 €
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