Name:
Phone:
E-mail:
Address:






eEF1B-alpha1 an 2 | elongation factor 1B-alpha 1 and 1B-alpha 2

AS10 678 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana

eEF1B-alpha1 an 2 | elongation factor 1B-alpha 1 and 1B-alpha 2 in the group Antibodies Plant/Algal  / DNA/RNA/Cell Cycle / Translation at Agrisera AB (Antibodies for research) (AS10 678)
eEF1B-alpha1 an 2 | elongation factor 1B-alpha 1 and 1B-alpha 2



DATA SHEET IN PDF

Qty: 
394
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

Recombinant eEF1B-alpha1 protein from Arabidopsis thaliana with no affinity tag,UniProt: Q84WM9, TAIR: At5g12110

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 3000 (WB)
Expected | apparent MW

24 | 34 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Glycne max, Oryza sativa, Solanum tuberosum, Zea mays, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples

Application example
western blotdetection using anti-elongation factor alpha 1 and 2 antibodies

10 µg total protein extracted from 15 day old Arabidopsis thaliana seedlings that were either kept at 22ºC (1) or heat treated at 38ºC for 2 hours prior to protein extraction (2). As positive control 10 ng of recombinant elongation factor proteins alpha 1 (3) and alpha 2 (4) were separated side by side with the plant samples on 11% SDS-PAGE and blotted to nitrocellulose (Bio-rad). Blots were blocked following transfer with 5% low fat milk in low salt buffer for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 2000 for 2h at room temperature with agitation in the blocking solution. The primary antibody solution was removed and the blot was rinsed briefly twice, then washed 4 times for 15 min each at room temperature with agitation using low salt buffer. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxides conjugated), diluted to 1:2500 for 1h at room temperature with agitation then washed as above and treated with ECL detection reagent according to the manufacturers instructions. Exposure time was 5 seconds. The primary antibody could be reused if it is kept at 4ºC for 2 weeks and if frozen at -20ºC for long time. The pre-immune did not cross react with any plant protein non-specifically. Low salt buffer components are 10 mM Tris (pH 7.6), 68 mM NaCl and 0.05 % Triton X-100.

Additional information

Additional information Antibody is recognizing elongation factor alpha 1 and alpha 2

Related products

Background

Background

eEF1B-alpha1 protein belongs to a family of elongation factors, proteins which are involved in translational elongation. This protein is a component of elongation factor B-complex which is composed of 2 elongation B-alpha-(1 and 2), 2 elongation factors B-beta (1 and 2) and 2 elongation factors B-gamma (1 and 2). Alternative names:

Product citations

Confirmed reactivity: Arabidopsis thaliana
predicted reactivity: Glycne max, Oryza sativa, Solanum tuberosum, Zea mays, Vitis vinifera
Species of your interest not listed? Contact us
not reactive in: No confirmed exceptions from predicted reactivity are currently known
background:

eEF1B-alpha1 protein belongs to a family of elongation factors, proteins which are involved in translational elongation. This protein is a component of elongation factor B-complex which is composed of 2 elongation B-alpha-(1 and 2), 2 elongation factors B-beta (1 and 2) and 2 elongation factors B-gamma (1 and 2). Alternative names:

calculated | apparent molecular mass [kDa]:

24 | 34 kDa

Clonality: Polyclonal
Format: Lyophilized
Host: Rabbit
immunogen:

Recombinant eEF1B-alpha1 protein from Arabidopsis thaliana with no affinity tag,UniProt: Q84WM9, TAIR: At5g12110

Purity: Serum
Quantity: 100 ĩl
recommended dilution: 1 : 3000 (WB)
Reconstitution: For reconstitution add 100 ĩl of sterile water
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Western blot (WB)
additional information: Antibody is recognizing elongation factor alpha 1 and alpha 2
Picture (footer):

Application example
western blotdetection using anti-elongation factor alpha 1 and 2 antibodies

10 µg total protein extracted from 15 day old Arabidopsis thaliana seedlings that were either kept at 22ºC (1) or heat treated at 38ºC for 2 hours prior to protein extraction (2). As positive control 10 ng of recombinant elongation factor proteins alpha 1 (3) and alpha 2 (4) were separated side by side with the plant samples on 11% SDS-PAGE and blotted to nitrocellulose (Bio-rad). Blots were blocked following transfer with 5% low fat milk in low salt buffer for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 2000 for 2h at room temperature with agitation in the blocking solution. The primary antibody solution was removed and the blot was rinsed briefly twice, then washed 4 times for 15 min each at room temperature with agitation using low salt buffer. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxides conjugated), diluted to 1:2500 for 1h at room temperature with agitation then washed as above and treated with ECL detection reagent according to the manufacturers instructions. Exposure time was 5 seconds. The primary antibody could be reused if it is kept at 4ºC for 2 weeks and if frozen at -20ºC for long time. The pre-immune did not cross react with any plant protein non-specifically. Low salt buffer components are 10 mM Tris (pH 7.6), 68 mM NaCl and 0.05 % Triton X-100.

Related products: eEF1B-alpha1 an 2 | elongation factor 1B-alpha 1 and 1B-alpha 2

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 26 €
AS10 677 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
394 €
AS09 607 |  Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)

10 % until...
206 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 ...
201 €