eEF1B-alpha1 an 2 | elongation factor 1B-alpha 1 and 1B-alpha 2

Product no: AS10 678

AS10 678 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana

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  • Product Info
  • Immunogen:

    Recombinant eEF1B-alpha1 protein from Arabidopsis thaliana with no affinity tag,UniProt: Q84WM9, TAIR: At5g12110

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 100 l
    Reconstitution: For reconstitution add 100 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 3000 (WB)
    Expected | apparent MW:

    24 | 34 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Glycne max, Oryza sativa, Solanum tuberosum, Zea mays, Vitis vinifera
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example
    western blotdetection using anti-elongation factor alpha 1 and 2 antibodies

    10 µg total protein extracted from 15 day old Arabidopsis thaliana seedlings that were either kept at 22ºC (1) or heat treated at 38ºC for 2 hours prior to protein extraction (2). As positive control 10 ng of recombinant elongation factor proteins alpha 1 (3) and alpha 2 (4) were separated side by side with the plant samples on 11% SDS-PAGE and blotted to nitrocellulose (Bio-rad). Blots were blocked following transfer with 5% low fat milk in low salt buffer for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 2000 for 2h at room temperature with agitation in the blocking solution. The primary antibody solution was removed and the blot was rinsed briefly twice, then washed 4 times for 15 min each at room temperature with agitation using low salt buffer. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxides conjugated), diluted to 1:2500 for 1h at room temperature with agitation then washed as above and treated with ECL detection reagent according to the manufacturers instructions. Exposure time was 5 seconds. The primary antibody could be reused if it is kept at 4ºC for 2 weeks and if frozen at -20ºC for long time. The pre-immune did not cross react with any plant protein non-specifically. Low salt buffer components are 10 mM Tris (pH 7.6), 68 mM NaCl and 0.05 % Triton X-100.

  • Additional Information
  • Additional information: Antibody is recognizing elongation factor alpha 1 and alpha 2
  • Background
  • Background:

    eEF1B-alpha1 protein belongs to a family of elongation factors, proteins which are involved in translational elongation. This protein is a component of elongation factor B-complex which is composed of 2 elongation B-alpha-(1 and 2), 2 elongation factors B-beta (1 and 2) and 2 elongation factors B-gamma (1 and 2). Alternative names:

  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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