EIN3 | Ethylene insensitive 3

Product no: AS19 4273

AS19 4273  | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

Replaced by AS23 4896. Interested to receive free sample? Inquire. 

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  • Product Info
  • Immunogen: Recombinant EIN3 of Arabidopsis thaliana, UniProt: O24606, TAIR: At3g20770
    Host:

    Rabbit

    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 µl
    Reconstitution: For reconstitution add 50 µl, of sterile water
    Storage: Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 71.4 | 78 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Populus trichocarpa
    Not reactive in: Medicago truncatula
  • Additional Information
  • Additional information (application): Western blot condtions for anti-EIN3 antibodies are described in Smalle et al. 2002.
    Recommended membrane for blocking: nitrocellulose, blocking reagent: 10 % non-fat milk, antibody incubation buffer: PBS-T.
  • Background
  • Background: EIN3 (Ethylene insensitive 3) acts as a positive regulator in the ethylene response pathway and is required for ethylene responsiveness in adult plant tissues. Activated by phosphorylation by MPK3 and MPK6 (PubMed:18273012). Down-regulated by KIN10 that controls its protein stability under a phosphorylation-dependent manner.

    Alternative names: Protein ethylene insensitive 3.
  • Product Citations
  • Selected references: Park et al. (2023) Ethylene-triggered subcellular trafficking of CTR1 enhances the response to ethylene gas. Nat Commun. 2023;14(1):365. Published 2023 Jan 23. doi:10.1038/s41467-023-35975-6
    Gagne et al. (2004). Arabidopsis EIN3-binding F-box 1 and 2 form ubiquitin-protein ligases that repress ethylene action and promote growth by directing EIN3 degradation. Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6803-8.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts


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