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EIN3 | Ethylene insensitive 3

AS19 4273  | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

EIN3 | Ethylene insensitive 3 in the group Antibodies for Plant/Algal  / Hormones / Ethylene at Agrisera AB (Antibodies for research) (AS19 4273)

DATA SHEET IN PDF

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How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen Recombinant EIN3 of Arabidopsis thaliana , UniProt: O24606-1, TAIR: At3g20770
Host

Rabbit

Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 71.4 | 78 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Not reactive in Medicago truncatula

Application examples

Additional information

Western blot condtions for anti-EIN3 antibodies are described in Smalle et al. 2002.
Recommended membrane for blocking: nitrocellulose, blocking reagent: 10 % non-fat milk, antibody incubation buffer: PBS-T. 

Related products

Related products

AS12 1865 | Anti-EIN2 | Ethylene insensitive 2, rabbit antibodies
AS06 193 | Anti-IAA | indole 3 acetic acid, rabbit antibodies
AS11 1749 | IAA | auxin ELISA quantitation kit

antibodies to proteins involved in ethylene metabolism

Secondary antibodies

Background

Background EIN3 (Ethylene insensitive 3) acts as a positive regulator in the ethylene response pathway and is required for ethylene responsiveness in adult plant tissues. Activated by phosphorylation by MPK3 and MPK6 (PubMed:18273012). Down-regulated by KIN10 that controls its protein stability under a phosphorylation-dependent manner.

Alternative names: Protein ethylene insensitive 3.

Product citations

Selected references Gagne et al. (2004). Arabidopsis EIN3-binding F-box 1 and 2 form ubiquitin-protein ligases that repress ethylene action and promote growth by directing EIN3 degradation. Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6803-8.

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