EIN3 | Ethylene insensitive 3
AS19 4273 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Product Information
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
50 µl
Reconstitution
For reconstitution add 50 µl, of sterile water
Storage
Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
71,4 | 78 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Populus trichocarpa
Not reactive in
Medicago truncatula
Application examples
Additional information
Western blot condtions for anti-EIN3 antibodies are described in Smalle et al. 2002.
Recommended membrane for blocking: nitrocellulose, blocking reagent: 10 % non-fat milk, antibody incubation buffer: PBS-T.
Recommended membrane for blocking: nitrocellulose, blocking reagent: 10 % non-fat milk, antibody incubation buffer: PBS-T.
Background
Background
EIN3 (Ethylene insensitive 3) acts as a positive regulator in the ethylene response pathway and is required for ethylene responsiveness in adult plant tissues. Activated by phosphorylation by MPK3 and MPK6 (PubMed:18273012). Down-regulated by KIN10 that controls its protein stability under a phosphorylation-dependent manner.
Alternative names: Protein ethylene insensitive 3.
Alternative names: Protein ethylene insensitive 3.
Product citations
Selected references
Gagne et al. (2004). Arabidopsis EIN3-binding F-box 1 and 2 form ubiquitin-protein ligases that repress ethylene action and promote growth by directing EIN3 degradation. Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6803-8.
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