EIN3 | Ethylene insensitive 3

AS19 4273 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Product Information

Immunogen Recombinant EIN3 of Arabidopsis thaliana , UniProt: O24606, TAIR: At3g20770

Host

Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl, of sterile water

Storage Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW 71,4 | 78 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Populus trichocarpa

Not reactive in Medicago truncatula

Application examples

Additional information

Western blot condtions for anti-EIN3 antibodies are described in Smalle et al. 2002.
Recommended membrane for blocking: nitrocellulose, blocking reagent: 10 % non-fat milk, antibody incubation buffer: PBS-T.

Related products

Secondary antibodies

Background

Background EIN3 (Ethylene insensitive 3) acts as a positive regulator in the ethylene response pathway and is required for ethylene responsiveness in adult plant tissues. Activated by phosphorylation by MPK3 and MPK6 (PubMed:18273012). Down-regulated by KIN10 that controls its protein stability under a phosphorylation-dependent manner.

Alternative names: Protein ethylene insensitive 3.

Product citations

Selected references Gagne et al. (2004). Arabidopsis EIN3-binding F-box 1 and 2 form ubiquitin-protein ligases that repress ethylene action and promote growth by directing EIN3 degradation. Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6803-8.