EIN2 | Ethylene insensitive 2
AS12 1865 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Product Information
Immunogen
KLH-conjugated peptide chosen from EIN2 of Arabidopsis thaliana, UniProt: Q9S814, TAIR: AT5G03280
Chosen peptide is not to be found in EIN3.
Chosen peptide is not to be found in EIN3.
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
50 ĩg
Reconstitution
For reconstitution add 50 ĩl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 2000 (WB)
Expected | apparent MW
140 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Brachypodium distachyon, Cucumis sativus, Glycine max, Hordeum vulgare, Medicago truncatula, Nicotiana tabacum, Solanum lycopersicum, Oryza sativa, Phtheirospermum japonicum, Populus trichocarpa, Ricinus communis, Solanum lycopresicum, Sorghum bicolor, Triticum aestivum, , Zea mays, Vitis vinifera
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
Application example

40-60 µg of membrane protein (measured with Millipore Direct Detect spec.) isolated following membrane extraction buffer protocol from Dong et al. 2008 (Plant Journal 53(2): 275-286) from 4 day old dark grown Arabidopsis thaliana seedlings, grown on MS plated with ACC or AVG in the plate, extracted with (10 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 10 % glycerol, 1 % Triton X-100, and protease ihibitors cocktail from Sigma) were separated 4-20% Bio-Rad precast SDS-PAGE gel and blotted 1h to PVDF membrane using semi-dry transfer. Blots were blocked with 5% milk for 2h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was then washed 5 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:20 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with Bio-Rad ECL according to the manufacturer's instructions. Exposure time was 25 min (to see full length EIN2).
Antibody is suitable for detection of full length EIN2. Bands below 140 kDa are non-specific.
Courtesy of Jennifer Marie Shemansky, University of Maryland, USA

40-60 µg of membrane protein (measured with Millipore Direct Detect spec.) isolated following membrane extraction buffer protocol from Dong et al. 2008 (Plant Journal 53(2): 275-286) from 4 day old dark grown Arabidopsis thaliana seedlings, grown on MS plated with ACC or AVG in the plate, extracted with (10 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 10 % glycerol, 1 % Triton X-100, and protease ihibitors cocktail from Sigma) were separated 4-20% Bio-Rad precast SDS-PAGE gel and blotted 1h to PVDF membrane using semi-dry transfer. Blots were blocked with 5% milk for 2h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was then washed 5 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:20 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with Bio-Rad ECL according to the manufacturer's instructions. Exposure time was 25 min (to see full length EIN2).
Antibody is suitable for detection of full length EIN2. Bands below 140 kDa are non-specific.
Courtesy of Jennifer Marie Shemansky, University of Maryland, USA
Additional information
Background
Background
EIN2 (Ethylene-insensitive protein 2) is a central factor in signaling pathways regulated by ethylene including various processes like: plant defense and development, senescence, nucleotide sugar flux and tropisms. Alternative names: EIN2, ETHYLENE INSENSITIVE 2, PIR2, CKR1, CYTOKININ RESISTANT 1, ERA3, ENHANCED RESPONSE TO ABA3, ORE3, ORESARA 3, ORE2, ORESARA 2, ATEIN2.
Product citations
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