EIN2 | Ethylene insensitive 2

Product no: AS12 1865

AS12 1865 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen: KLH-conjugated peptide chosen from EIN2 of Arabidopsis thaliana, UniProt: Q9S814, TAIR: AT5G03280
    Chosen peptide is not to be found in EIN3.
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 2000 (WB)
    Expected | apparent MW:

    140 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Brachypodium distachyon, Cucumis sativus, Glycine max, Hordeum vulgare, Medicago truncatula, Nicotiana tabacum, Solanum lycopersicum, Oryza sativa, Phtheirospermum japonicum, Populus trichocarpa, Ricinus communis, Solanum lycopresicum, Sorghum bicolor, Triticum aestivum, , Zea mays, Vitis vinifera
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    western blot using anti-EIN2 antibodies

    40-60 µg of membrane protein (measured with Millipore Direct Detect spec.) isolated following membrane extraction buffer protocol from Dong et al. 2008 (Plant Journal 53(2): 275-286) from 4 day old dark grown Arabidopsis thaliana seedlings, grown on MS plated with ACC or AVG in the plate, extracted with (10 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 10 % glycerol, 1 % Triton X-100, and protease ihibitors cocktail from Sigma) were separated 4-20% Bio-Rad precast SDS-PAGE gel and blotted 1h to PVDF membrane using semi-dry transfer. Blots were blocked with 5% milk for 2h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was then washed 5 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:20 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with Bio-Rad ECL according to the manufacturer's instructions. Exposure time was 25 min (to see full length EIN2).

    Antibody is suitable for detection of full length EIN2. Bands below 140 kDa are non-specific.

    Courtesy of Jennifer Marie Shemansky, University of Maryland, USA
  • Background
  • Background:

    EIN2 (Ethylene-insensitive protein 2) is a central factor in signaling pathways regulated by ethylene including various processes like: plant defense and development, senescence, nucleotide sugar flux and tropisms. Alternative names: EIN2, ETHYLENE INSENSITIVE 2, PIR2, CKR1, CYTOKININ RESISTANT 1, ERA3, ENHANCED RESPONSE TO ABA3, ORE3, ORESARA 3, ORE2, ORESARA 2, ATEIN2.

  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts


    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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