Enolase 2

AS10 651 | Clonality:Polyclonal | Host:Rabbit | Reactivity: Arabidopsis thaliana, Helianthus annuus

Enolase 2 in the group Antibodies Plant/Algal  / Carbohydrates at Agrisera AB (Antibodies for research) (AS10 651)
Enolase 2


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Product name, number (Agrisera, Sweden)

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Product Information


Recombinant  Arabidopsis thaliana enolase UniProt: P25696-1, TAIR: At2g36530

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW

47.7 kDa (Arabidopsis thaliana)


Confirmed reactivity Arabidopsis thaliana, Helianthus annuus
Predicted reactivity Brassica sp., Chlamydomonas reinhardii, Lycopersicum esculentum, Gossypium mexicanum, Nannochloropsis gaditana, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Populus balsamifera, Ricinus communis, Zea mays
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

Western blot using anti-enolase antibodies

Coomassie staining of three recombinant sunflower ENO proteins after purification on IMAC column and SDS PAGE separation (A) Immunodetection carried out with the anti-Enolase antibody (B) (AS10 651 at 1:2000 dilution). The detection was done with the Goat Anti-Rabbit IgG (H+L) Alkaline phosphatase conjugated (AS09 607 at 1:5000 dilution). In (A) and (B), the lanes were loaded as follows: M indicates the molecular weight markers Lane 1- Recombinant (6xHis)HaENO2 (cytosolic and active isoform) Lane 2- Recombinant (6xHis)ΔHaENO1 (plastidial isoform with the N-terminal transit peptide removed) Lane 3- Recombinant (6xHis)HaENO3 (cytosolic and inactive isoform) In panel (A), 0.7 µg protein was loaded per lane. In panel (B) 50 ng protein was loaded per lane. The faint band seen below the main band in lane 1 in (B) is likely a degradation product of the recombinant protein. No band was detected in lanes 2 and 3.

Recombinant sunflower enolases are described Troncoso-Ponce et al. Plant Science (2018) 272:117-130).

Courtesy of Dr. Jean Rivoal, IRBV, Université de Montréal, Canada

Additional information

Antibody is specific for the ENO2 isoform (cytosolic and active isoform), see data below

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Plant protein extraction buffer

Secondary antibodies



Enolase (2-phospho-D-glycerate hydrolase or phosphopyruvate dehydratase) is an essential glycolytic metalloenzyme. It is catalyzing the interconversion of 2-phosphoglycerate and phosphoenolpyruvate.

Alternative name: Bifunctional enolase 2/transcriptional activator 

Product citations

Selected references Zhang et al. (2020). A moonlighting role for enzymes of glycolysis in the co-localization of mitochondria and chloroplasts. Nat Commun. 2020 Sep 9;11(1):4509.doi: 10.1038/s41467-020-18234-w.
Zhang et al. (2018). Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ. 2017 Sep;40(9):1834-1848. doi: 10.1111/pce.12989.
Chen et al.(2009) System analysis of an Arabidopsis mutant altered in de novo fatty acid synthesis reveals diverse changes in seed composition and metabolic regulation. Plant Physiol.

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