EPSP synthase | 3-phosphoshikimate 1-carboxyvinyltransferase (chloroplastic)

AS16 3119 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Amaranthus palmeri

Product Information

Immunogen

KLH-conjugated peptide, derived from Amaranthus palmeri EPSP synthase, UniProt: M1K439

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 2000-1 : 5000 (WB)

Expected | apparent MW

55 kDa

Reactivity

Confirmed reactivity Amaranthus palmeri

Predicted reactivity Asimina triloba, Erigeron annuus, Chlamydomonas reinhardtii, Genlisea aurea, Gossypium raimondii, Lolium rigidum, Musa acuminata, Nannochloropsis gaditana, Nicotiana tabacum, Nicotiana sylvestris, Oryza sativa subsp. indica, Plantago lanceolata, Sorghum halepense, Zea mays, Vitis vinifera
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Application examples

Additional information

Related products

AS14 2782 | Anti-EPSP synthase | 3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic, rabbit antibodies

Recommended secondary antibody for ECL detection

Plant protein extraction buffer

Secondary antibodies

Background

Background EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic) is an enzyme (EC:2.5.1.19)which is involved in glyphosate metabolic process. Localized in chloroplast stroma. Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate. Alternative name: 5-enolpyruvylshikimate-3-phosphate synthase.

Product citations

Selected references Fernández-Escalada et al. (2016). Characterization of the Amaranthus palmeri Physiological Response to Glyphosate in Susceptible and Resistant Populations. J Agric Food Chem. 64 (1): 95-106. doi: 10.1021/acs.jafc.5b04916.