EPSP synthase | 3-phosphoshikimate 1-carboxyvinyltransferase (chloroplastic, higher plants)

AS16 3119 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Amaranthus palmeri


product information
Background		EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic) is an enzyme (EC:2.5.1.19)which is involved in glyphosate metabolic process. Localized in chloroplast stroma. Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate. Alternative name: 5-enolpyruvylshikimate-3-phosphate synthase.
Immunogen		KLH-conjugated peptide, derived from Amaranthus palmeri EPSP synthase, UniProt: M1K439
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Lyophilized
Quantity		50 µl
Reconstitution		For reconstitution add 50 µl of sterile water.
Storage		Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		Western blot (WB)
Related products		AS14 2782 \| anti-EPSP synthase \| 3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic, rabbit antibodies Recommended secondary antibody for ECL detection Plant protein extraction buffer Secondary antibodies
Additional information

application information
Recommended dilution		1 : 2000-1 : 5000 (WB)
Expected \| apparent MW		55 kDa
Confirmed reactivity		Amaranthus palmeri
Predicted reactivity		Asimina triloba, Erigeron annuus, Genlisea aurea, Gossypium raimondii, Lolium rigidum, Musa acuminata, Nicotiana tabacum, Nicotiana sylvestris, Oryza sativa subsp. indica, Plantago lanceolata, Sorghum halepense, Zea mays, Vitis vinifera
Not reactive in
Additional information
Selected references		Fernández-Escalada et al. (2016). Characterization of the Amaranthus palmeri Physiological Response to Glyphosate in Susceptible and Resistant Populations. J Agric Food Chem. 64 (1): 95-106. doi: 10.1021/acs.jafc.5b04916.

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