Name:
Phone:
E-mail:
Address:






ExoS | Exoenzyme S

AS05 056 | Clonality: polyclonal | Host: Hen | Reactivity:  Pseudomonas aeruginosa

ExoS | Exoenzyme S in the group Antibodies Other Species / Bacteria at Agrisera AB (Antibodies for research) (AS05 056)



DATA SHEET IN PDF

Qty: 
424
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

amino acids 366 to 453 of PA3841 of ADP-rbosylating enzyme - Exoenzyme S overexpressed in a GST fusion. Afterwards cleaved with a help of trombin and separated on a polyacrylamide gel. Gel piece has been used for immunizations.

Host Chicken
Clonality Polyclonal
Purity Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
Format Liquid
Quantity 100 ĩl
Storage Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

48 kDa

Reactivity

Confirmed reactivity Pseudomonas aeruginosa
Predicted reactivity Pseudomonas aeruginosa
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Related products

Background

Background

Exoenzyme S (ExoS) is a toxin directly translocated into eukaryotic cells by the type III secretory process of Pseudomonas aeruginosa. It is a bi-functional toxin and contain an N-terminal Rho GAP domain that disrupts the actin cytoskeleton and interference of phagocytosis. The C-terminal of ExoS contains an ADP-ribosyltransferase domain, which elicits a cytotoxic phenotype in cultured cells.

Product citations

Selected references Feng et al. (2019: Tanshinones: First-in-Class Inhibitors of the Biogenesis of the Type 3 Secretion System Needle of Pseudomonas aeruginosa for Antibiotic Therapy. ACS Cent. Sci.2019.
Anantharajah et al. (2017). Salicylidene acylhydrazides and hydroxyquinolines act as inhibitors of type three secretion systems in Pseudomonas aeruginosa by distinct mechanisms. Antimicrob Agents Chemother. 2017 Apr 10. pii: AAC.02566-16. doi: 10.1128/AAC.02566-16.
Anantharajah et al. (2016). Inhibition of the Injectisome and Flagellar Type III Secretion Systems by INP1855 Impairs Pseudomonas aeruginosa Pathogenicity and Inflammasome Activation. J Infect Dis. 2016 Jul 13. pii: jiw295.
immunogen:

amino acids 366 to 453 of PA3841 of ADP-rbosylating enzyme - Exoenzyme S overexpressed in a GST fusion. Afterwards cleaved with a help of trombin and separated on a polyacrylamide gel. Gel piece has been used for immunizations.

Host: Chicken
Clonality: Polyclonal
Purity: Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
Format: Liquid
Quantity: 100 ĩl
storage: Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Western blot (WB)
recommended dilution: 1 : 5000 (WB)
calculated | apparent molecular mass [kDa]:

48 kDa

Confirmed reactivity: Pseudomonas aeruginosa
predicted reactivity: Pseudomonas aeruginosa
not reactive in: No confirmed exceptions from predicted reactivity are currently known
background:

Exoenzyme S (ExoS) is a toxin directly translocated into eukaryotic cells by the type III secretory process of Pseudomonas aeruginosa. It is a bi-functional toxin and contain an N-terminal Rho GAP domain that disrupts the actin cytoskeleton and interference of phagocytosis. The C-terminal of ExoS contains an ADP-ribosyltransferase domain, which elicits a cytotoxic phenotype in cultured cells.

All references: Feng et al. (2019: Tanshinones: First-in-Class Inhibitors of the Biogenesis of the Type 3 Secretion System Needle of Pseudomonas aeruginosa for Antibiotic Therapy. ACS Cent. Sci.2019.
Anantharajah et al. (2017). Salicylidene acylhydrazides and hydroxyquinolines act as inhibitors of type three secretion systems in Pseudomonas aeruginosa by distinct mechanisms. Antimicrob Agents Chemother. 2017 Apr 10. pii: AAC.02566-16. doi: 10.1128/AAC.02566-16.
Anantharajah et al. (2016). Inhibition of the Injectisome and Flagellar Type III Secretion Systems by INP1855 Impairs Pseudomonas aeruginosa Pathogenicity and Inflammasome Activation. J Infect Dis. 2016 Jul 13. pii: jiw295.

Related products: ExoS | Exoenzyme S

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 26 €
AS09 606 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Chicken IgY (H&L...
206 €
AS11 1827 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Chicken IgY (H&...
206 €
AS10 1489 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken IgG/Y (H&L)

10 % un...
206 €