Name:
Phone:
E-mail:
Message
AGO set
Tag Antibody Set 

ExoS | Exoenzyme S

AS05 056 | Clonality: polyclonal | Host: Hen | Reactivity:  Pseudomonas aeruginosa

ExoS | Exoenzyme S in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (AS05 056)

PRODUCT INFORMATION IN PDF

Qty: 
381 €

Datasheet Product citations Protocols Add review

Product Information

Immunogen

amino acids 366 to 453 of PA3841 of ADP-rbosylating enzyme - Exoenzyme S overexpressed in a GST fusion. Afterwards cleaved with a help of trombin and separated on a polyacrylamide gel. Gel piece has been used for immunizations.

Host Chicken
Clonality Polyclonal
Purity Total IgY
Format Liquid in PBS pH 8.0, 0.02% sodium azide
Quantity 100 µl
Storage Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

48 kDa

Reactivity

Confirmed reactivity Pseudomonas aeruginosa
Predicted reactivity Pseudomonas aeruginosa
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Related products

Related products IMS01-133-332 | Anti-Pseudomonas aeruginosa, chicken antibodies

Secondary antibodies

Background

Background

Exoenzyme S (ExoS) is a toxin directly translocated into eukaryotic cells by the type III secretory process of Pseudomonas aeruginosa. It is a bi-functional toxin and contain an N-terminal Rho GAP domain that disrupts the actin cytoskeleton and interference of phagocytosis. The C-terminal of ExoS contains an ADP-ribosyltransferase domain, which elicits a cytotoxic phenotype in cultured cells.

Product citations

Selected references Anantharajah et al. (2017). Salicylidene acylhydrazides and hydroxyquinolines act as inhibitors of type three secretion systems in Pseudomonas aeruginosa by distinct mechanisms. Antimicrob Agents Chemother. 2017 Apr 10. pii: AAC.02566-16. doi: 10.1128/AAC.02566-16.
Anantharajah et al. (2016). Inhibition of the Injectisome and Flagellar Type III Secretion Systems by INP1855 Impairs Pseudomonas aeruginosa Pathogenicity and Inflammasome Activation. J Infect Dis. 2016 Jul 13. pii: jiw295.

Related products: ExoS | Exoenzyme S

AS16 ECL-SN-10 | low pico to mid femtogram and extreme low femtogram detection
15 €
AS09 606 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Chicken IgY (H&L...
186 €
AS11 1827 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Chicken IgY (H&...
186 €
AS10 1489 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken IgG/Y (H&L)
186 €