E(z) | Histone-lysine N-methyltransferase E(z)

ChIP and qPCR analysis were done as described [Schwartz YB, Kahn TG, Nix DA, Li XY, Bourgon R, et al. (2006) Genome-wide analysis of Polycomb targets in Drosophila melanogaster. Nat Genet 38: 700–705. doi: 10.1038/ng1817]. Chromatin from Su(z)12 mutant cell line (Kahn T. et all, submitted) served as a negative control, chromatin from Ras3 cells served as a positive control. Quantitative PCR was performed with primers specific for BXD-PRE of Ubx gene (Polycomb target gene in repressed state), used as positive controls, and for intergenic region, used as negative control. Figure 1 shows the ChIP recovery, measured by qPCR as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA). Chromatin from 5x107 cells and 3mg of anti-E(z) antibody were used for each ChIP reaction.

Courtesy of Dr. Tatyana Khan, Umeå University, Sweden.

Secondary antibodies