E(z) | Histone-lysine N-methyltransferase E(z)
AS16 3935 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Drosophila melanogaster
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Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Figure 1. ChIP recovery.
ChIP and qPCR analysis were done as described [Schwartz YB, Kahn TG, Nix DA, Li XY, Bourgon R, et al. (2006) Genome-wide analysis of Polycomb targets in Drosophila melanogaster. Nat Genet 38: 700–705. doi: 10.1038/ng1817]. Chromatin from Su(z)12 mutant cell line (Kahn T. et all, submitted) served as a negative control, chromatin from Ras3 cells served as a positive control. Quantitative PCR was performed with primers specific for BXD-PRE of Ubx gene (Polycomb target gene in repressed state), used as positive controls, and for intergenic region, used as negative control. Figure 1 shows the ChIP recovery, measured by qPCR as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA). Chromatin from 5x107 cells and 3mg of anti-E(z) antibody were used for each ChIP reaction.
Courtesy of Dr. Tatyana Khan, Umeå University, Sweden.
Alternative names: Lysine N-methyltransferase 6, Protein enhancer of zeste
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