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Fd1, Fd2, Fd3, Fd4 | Ferredoxin 1,2,3,4

AS20 4431 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Arabidopsis thaliana, Zea mays
Fd1, Fd2, Fd3, Fd4  | Ferredoxin 1,2,3,4 in the group Antibodies for Plant/Algal  / Photosynthesis  / Electron transfer at Agrisera AB (Antibodies for research) (AS20 4431)

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Product Information

Immunogen Native, chromafography purified ferredoxin mixture: Fd1, Fd2, Fd3 and Fd4 from Zea mays, UniProt: P27787 (Fd1), P27787(Fd2), P27788 (Fd3), accesion number not known for Fd4
Host Rabbit
Clonality Polyclonal
Purity Total IgG, purified on Protein A
Quantity 100 µg
Storage Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1: 1000 - 1: 10 000 (WB)
Expected | apparent MW 12 | 16-17 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Zea mays
Predicted reactivity plant ferredoxin isoforms
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Western blot using anti-global plant ferredoxin antibodies


10 µg/well of Arabidopsis thaliana total leaf extract (1), 10 µg/well Zea mays total leaf extract (2) freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE. For IP, 150mM NaCL, 1% Triton X-100, 50 mM Tris-HCl (pH 8.0) and denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.

Additional information

Following processing MW of plant ferredoxins is around 12 kDa. However, due to a strong acidic nature, they migrate at 16 to 17 kDa.

Related products

Related products AS20 4434 | Anti-Fd1 | Ferredoxin 1 (chloroplastic), rabbit antibodies
AS20 4433 | Anti-Fd2 | Ferredoxin 2 (chloroplastic), rabbit antibodies
AS20 4432 | Anti-Fd3 | Ferredoxin 3, rabbit antibodies
collection of antibodies involved in photosynthesis

Background

Background Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Occupies a key position both for transferring the photoreducing power to Fd-NADP+ oxidoreductase (FNR), hence the formation of NADPH, and for mediating the cyclic electron flow around photosystem I (PSI).

Product citations

Selected references Hase et al. (1991). Molecular Cloning and Differential Expression of the Maize Ferredoxin Gene Family. Plant Physiol. 96(1):77-83. doi: 10.1104/pp.96.1.77.

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