IKP

FdC1 | Ferredoxinx-C1

Product no: AS20 4430
AS20 4430 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Arabidopsis thaliana, Zea mays
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  • Product Info
  • Immunogen: Purified full length, tag cleaved, recombinant Arabidopsis thaliana Ferredoxin C-1, UniProt: O23344 , TAIR: At4g14890
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
    Format: Liquid at 2 mg/ml.
    Quantity: 100 g
    Storage: Store at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Western blot (WB)
    Recommended dilution: 1: 1000 - 1: 5000 (WB)
    Expected | apparent MW: 16,7 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Zea mays
    Predicted reactivity: Brassica rapa, Cannabis sativa, Theobroma cacao
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Western blot using anti-Ferredoxin-C1 antibodies

    10 µg of Arabidopsis thaliana total leaf extract (1), 10 µg of Zea mays total leaf extract (2) were freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE. For IP, 150mM NaCL, 1% Triton X-100, 50 mM Tris-HCl (pH 8.0) and denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.
  • Background
  • Background: Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Higher plants also possess genes for significantly different, as yet uncharacterized Fd proteins, with extended C termini (FdCs). Whether these FdC proteins function as photosynthetic electron transfer proteins is not known. It has been suggested that FdC1 has a specific function in conditions of acceptor limitation at PSI, and channels electrons away from NADP(+) photoreduction.
  • Product Citations
  • Selected references: Voss et al. (2011). FdC1, a Novel Ferredoxin Protein Capable of Alternative Electron Partitioning, Increases in Conditions of Acceptor Limitation at Photosystem I. J Biol Chem. 2011 Jan 7;286(1):50-9. doi: 10.1074/jbc.M110.161562.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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