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FtsZ2 | Plant cell division protein ftsZ2

AS13 2651| Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

FtsZ2 | Plant cell division protein ftsZ2 in the group Antibodies for Plant/Algal  / Developmental Biology / Cytoskeleton at Agrisera AB (Antibodies for research) (AS13 2651)

DATA SHEET IN PDF

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Product Information

Immunogen

Recombinant part of Arabidopsis thalina FtsZ2 conserved in FtsZ2-1 UniProt: O82533, TAIR: At2g36250, FtsZ2-2 UniProt: Q9LXJ0, TAIR: AT3G52750

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW

50.7 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare
Predicted reactivity Glycne max, Jatropha manihot, Ricinus communis
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example


2 µg of recombinant FtsZ2 fragment(~10 kDa) (1) and total protein extracted with PEB AS08 300 from leaf tissue Arabidopsis thaliana (2) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with FtsZ2 antibody (AS13 2651, 1:2000, 1h) and secondary anti-rabbit (1:25 000, 1 h) antibody (HRP conjugated, secondary antibody AS09 602 in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. The signal was developed for 5 min with ECL according to the manufacturer’s instructions. Exposure time was 2 min.

Additional information

Additional information Antibody available in 3 various pack sizes: 50, 100 and 150 µl - please, inquire.

Related products

Related products

AS07 217 | Anti-FtsZ | procaryotic cell division GTPase, cyanobacterial, rabbit antibodies
AS09 413 | Anti-FtsZ1 and 2 | plant cell division protein ftsZ1 and ftsZ2 , rabbit antibodies
AS10 715 | Anti-FtsZ procaryotic cell division GTPase (bacterial), rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Background

FtsZ2 belongs to FtsZ family and binds to FtsZ1 and exhibits  GTPase activity. Component of a plastid division machinery.

Alternative names: Plastid division protein FTSZ2-1, AtFtsZ2-1, Plastid division protein FTSZ2-2, AtFtsZ2-2.

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