FtsZ2 | Plant cell division protein ftsZ2

Name: FtsZ2 | Plant cell division protein ftsZ2
SKU: AS13 2651
Price: 302 EUR
Availability: InStock

AS13 2651| Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Benefits of using this antibody

FtsZ2 | Plant cell division protein ftsZ2 in the group Antibodies Plant/Algal / Plant Developmental Biology / Cytoskeleton at Agrisera AB (Antibodies for research) (AS13 2651)

FtsZ2 | Plant cell division protein ftsZ2

Product Information

Immunogen

Recombinant part of Arabidopsis thalina FtsZ2 conserved in FtsZ2-1 UniProt: O82533, TAIR: At2g36250, FtsZ2-2 UniProt: Q9LXJ0, TAIR: AT3G52750

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 2000 (WB)

Expected | apparent MW 50,7 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare

Predicted reactivity Glycne max, Jatropha manihot, Ricinus communis
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

2 µg of recombinant FtsZ2 fragment(~10 kDa) (1) and total protein extracted with PEB AS08 300 from leaf tissue Arabidopsis thaliana (2) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with FtsZ2 antibody (AS13 2651, 1:2000, 1h) and secondary anti-rabbit (1:25 000, 1 h) antibody (HRP conjugated, secondary antibody AS09 602 in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. The signal was developed for 5 min with ECL according to the manufacturer’s instructions. Exposure time was 2 min.

Additional information

Additional information Antibody available in 3 various pack sizes: 50, 100 and 150 µl - please, inquire

Background

FtsZ2 belongs to FtsZ family and binds to FtsZ1 and exhibits GTPase activity. Component of a plastid division machinery.

Alternative names: Plastid division protein FTSZ2-1, AtFtsZ2-1, Plastid division protein FTSZ2-2, AtFtsZ2-2.

Product citations

immunogen:	Recombinant part of Arabidopsis thalina FtsZ2 conserved in FtsZ2-1 UniProt: O82533, TAIR: At2g36250, FtsZ2-2 UniProt: Q9LXJ0, TAIR: AT3G52750
Reconstitution:	For reconstitution add 50 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 µl
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 2000 (WB)
calculated \| apparent molecular mass [kDa]:	50,7 kDa
Confirmed reactivity:	Arabidopsis thaliana, Hordeum vulgare
predicted reactivity:	Glycne max, Jatropha manihot, Ricinus communis Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	application example 2 µg of recombinant FtsZ2 fragment(~10 kDa) (1) and total protein extracted with PEB AS08 300 from leaf tissue Arabidopsis thaliana (2) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with FtsZ2 antibody (AS13 2651, 1:2000, 1h) and secondary anti-rabbit (1:25 000, 1 h) antibody (HRP conjugated, secondary antibody AS09 602 in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. The signal was developed for 5 min with ECL according to the manufacturer’s instructions. Exposure time was 2 min.
additional information:	Antibody available in 3 various pack sizes: 50, 100 and 150 µl - please, inquire
background:	FtsZ2 belongs to FtsZ family and binds to FtsZ1 and exhibits GTPase activity. Component of a plastid division machinery. Alternative names: Plastid division protein FTSZ2-1, AtFtsZ2-1, Plastid division protein FTSZ2-2, AtFtsZ2-2.