FtsZ2 | Plant cell division protein ftsZ2
AS13 2651| Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Recombinant part of Arabidopsis thalina FtsZ2 conserved in FtsZ2-1 UniProt: O82533, TAIR: At2g36250, FtsZ2-2 UniProt: Q9LXJ0, TAIR: AT3G52750
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2 µg of recombinant FtsZ2 fragment(~10 kDa) (1) and total protein extracted with PEB AS08 300 from leaf tissue Arabidopsis thaliana (2) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with FtsZ2 antibody (AS13 2651, 1:2000, 1h) and secondary anti-rabbit (1:25 000, 1 h) antibody (HRP conjugated, secondary antibody AS09 602 in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. The signal was developed for 5 min with ECL according to the manufacturer’s instructions. Exposure time was 2 min.
FtsZ2 belongs to FtsZ family and binds to FtsZ1 and exhibits GTPase activity. Component of a plastid division machinery.
Alternative names: Plastid division protein FTSZ2-1, AtFtsZ2-1, Plastid division protein FTSZ2-2, AtFtsZ2-2.
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