FtsZ | Procaryotic cell division GTPase
AS10 715 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Candidatus Moranella endobia PCIT , Escherichia DH5a, Escherichia coli BW 25113, Shigella flexneri
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40 | 42 kDa
Candidatus sp., Citrobacter sp. 30_2, Dickeya sp.,
B. subtilis, Listera sp., Neisseria meningitidis, Pseudomonas aeruginosa, Staphylococcus aureus (strain MRSA252), cyanobacteria
5 µg of total protein from Synechocystis sp. (1), E.coli DH5a (2), E. coli (3), extracted with Agrisera PEB extraction buffer were separated on 4-12% SDS-PAGE and blotted 1h to PVDF. Blots were blocked with Advance blocking reagent for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 120 seconds.
Total protein extract from E. coli CFT073: 8µg (1), 12µg (2), 16µg (3). Proteins were separated on 10% SDS-PAGE and blotted to PVDF. Blocked with 5 % non-fat milk in TBS-T for 1 hour. Blot was incubated in the primary antibody at a dilution of 1 :10 000 for 1 h at RT with agitation. Secondary antibody (anti-rabbit IgG, HRP conjugated, Agrisera, AS09 602) were diluted to 1 : 50 000 and blot was incubated for 1h at RT with agitation. Immunodetection was performed using chemiluminescent detection method for 3 min. Scan was made after 30 sec.
Courtesy of Dr. Marta Kicia, Wroclaw Medical University, Poland
FtsZ (cell division GTPase) is a well characterized protein of the bacterial cell division apparatus. This protein accumulates early in dividing cells, and has a crucial role during septum formation in most bacteria. It has also been accepted as the bacterial cytoskeletal counterpart to eukaryotic microtubules. Synonymes: sifB, SulB.
Ranjit et al. (2020). Chlamydial MreB Directs Cell Division and Peptidoglycan Synthesis in Escherichia coli in the Absence of FtsZ Activity. mBio. 2020 Feb 18;11(1). pii: e03222-19. doi: 10.1128/mBio.03222-19. (Immunofluorescence)
Sekar et al. (2018). Synthesis and degradation of FtsZ quantitatively predict the first cell division in starved bacteria. Mol Syst Biol. 2018 Nov 5;14(11):e8623. doi: 10.15252/msb.20188623.
Mückl et al. (2018). Filamentation and restoration of normal growth in Escherichia coli using a combined CRISPRi sgRNA/antisense RNA approach. PLoS One. 2018 Sep 11;13(9):e0198058. doi: 10.1371/journal.pone.0198058. eCollection 2018.
Pende et al. (2014). Size-independent symmetric division in extraordinarily long cells. Nat Commun. 2014 Sep 15;5:4803. doi: 10.1038/ncomms5803.
Söderström et al. (2014). Disassembly of the divisome in Escherichia coli: Evidence that FtsZ dissociates before compartmentalisation. Mol Microbiol. 2014 Feb 7. doi: 10.1111/mmi.12534. (western blot and immunofluorescence)
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