FtsZ | Procaryotic cell division GTPase

KLH-conjugated synthetic peptide derived from known bacterial sequences of FtsZ including E.coli UniProt: P0A9A6

40 | 42 kDa

Candidatus sp., Citrobacter sp. 30_2, Dickeya sp., Species of your interest not listed? Contact usm>Enterobacter sp., Klebsiella pneumoniae subsp. pneumoniae MGH, Salmonella sp., Shigella sonnei Ss046, Vibrio sp., Yersinia pestis D182038

B. subtilis, Listera sp., Neisseria meningitidis, Pseudomonas aeruginosa, Staphylococcus aureus (strain MRSA252), cyanobacteria

 5 µg of total protein from Synechocystis sp. (1), E.coli DH5a (2), E. coli (3),  extracted with Agrisera PEB extraction buffer were separated on  4-12% SDS-PAGE and blotted 1h to PVDF. Blots were blocked with Advance blocking reagent  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 120 seconds.

Total protein extract from E. coli CFT073: 8µg (1), 12µg (2), 16µg (3). Proteins were separated on 10% SDS-PAGE and blotted to PVDF. Blocked with 5 % non-fat milk in TBS-T for 1 hour. Blot was incubated in the primary antibody at a dilution of 1 :10 000 for 1 h at RT with agitation. Secondary antibody (anti-rabbit IgG, HRP conjugated, Agrisera, AS09 602) were diluted to 1 : 50 000 and blot was incubated for 1h at RT with agitation. Immunodetection was performed using chemiluminescent detection method for 3 min. Scan was made after 30 sec.

Courtesy of Dr. Marta Kicia, Wroclaw Medical University, Poland

AS07 217 | Anti-FtsZ | procaryotic cell division GTPase, cyanobacterial, rabbit antibodies
AS09 413 | Anti-FtsZ1 and 2 | plant cell division protein ftsZ1 and ftsZ2 , rabbit antibodies
AS13 2651 | Anti-FtsZ2 plant cell division protein ftsZ2, rabbit antibodies

Secondary antibodies

FtsZ (cell division GTPase) is a well characterized protein of the bacterial cell division apparatus. This protein accumulates early in dividing cells, and has a crucial role during septum formation in most bacteria. It has also been accepted as the bacterial cytoskeletal counterpart to eukaryotic microtubules. Synonymes: sifB, SulB.