Anti-FtsZ1 and 2 | Plant cell division protein FtsZ1 and FtsZ2

Product no: AS09 413

AS09 413 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thalian, Hordeum vulgare

Benefits of using this antibody

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  • Product Info
  • Immunogen:

    Recombinant part of Arabidopsis thalina FtsZ conserved in FtsZ1 Q42545 At5g55280 and FtsZ2 including FtsZ2-1 O82533, At2g36250 and FtsZ2-2 Q9LXJ0, At3g52750 and in a wide range of FtsZ proteins from other plant species.

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 2500 (WB)
    Expected | apparent MW:

    40 kDa FtsZ1 (Arabidopsis thaliana) and 50 kDa FtsZ2 (Arabidopsis thaliana)

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Hordeum vulgare
    Predicted reactivity: Chlamydomons reinhardtii, Cucumis sativus, Gentiana lutea, Glycine max, Gossypium arobretum, Jatropha manihot, Lilium longiflorum, Lupinus angustifolius, Manihot esculenta, Marchantia aquatica, Medicago truncatula, Morus notabilis, Nannochloropsis gaditana, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, populus trichocarpa, Ricinus communis, Solanum lycopersicum, Sorgum bicolor, Theobroma cacao, Triticum uRatum, Zea mays, Yellow gentian, Vitis vinifera
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    western blot using plant anti-FtsZ1 and FtsZ2 antibody

    2 µg
    of total protein extracted with PEB (AS08 300) from leaf tissue of (1) Hordeum vulgare, (2) Arabidopsis thaliana, (3 - 5) recombinant FtsZ1/FtsZ2 (33 kDa) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-FtsZ1/FtsZ2 antibody (AS09 413, 1:2500, 1h) and secondary anti-rabbit (1:10 000, 1 h) antibody (HRP conjugated, secondary antibody AS09 602) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent, using a Fuji LAS-3000 CCD (300s, standard sensitivity). Exposure time was 2 min.
  • Additional Information
  • Additional information: This product can be sold containing proclin if requested
  • Background
  • Background:

    FtsZ1 (cell division protein FtsZ homolog 1) is required for plastid cell division. Localization: pollen grain and plastids of vegetative cells. Alternative names: Chloroplast FtsZ, Protein accumulation and replication of chloroplasts 10, Protein plastid movement impaired4.

    FtsZ2 (cell visision protein FtsZ homolog 2) is required for plastid cell division. Present in two isoforms: FtsZ2-1 and FtsZ2-2. Alternative names: Plastid division protein FTSZ2.

  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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AS13 2651 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Benefits of using this antibody

319 €
Add to cart
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items