FtsZ1 and 2 | Plant cell division protein FtsZ1 and FtsZ2
AS09 413 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thalian, Hordeum vulgare
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Recombinant part of Arabidopsis thalina FtsZ conserved in FtsZ1 Q42545 At5g55280 and FtsZ2 including FtsZ2-1 O82533, At2g36250 and FtsZ2-2 Q9LXJ0, At3g52750 and in a wide range of FtsZ proteins from other plant species.
40 kDa FtsZ1 (Arabidopsis thaliana) and 50 kDa FtsZ2 (Arabidopsis thaliana)
2 µg of total protein extracted with PEB (AS08 300) from leaf tissue of (1) Hordeum vulgare, (2) Arabidopsis thaliana, (3 - 5) recombinant FtsZ1/FtsZ2 (33 kDa) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-FtsZ1/FtsZ2 antibody (AS09 413, 1:2500, 1h) and secondary anti-rabbit (1:10 000, 1 h) antibody (HRP conjugated, secondary antibody AS09 602) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent, using a Fuji LAS-3000 CCD (300s, standard sensitivity). Exposure time was 2 min.
FtsZ1 (cell division protein FtsZ homolog 1) is required for plastid cell division. Localization: pollen grain and plastids of vegetative cells. Alternative names: Chloroplast FtsZ, Protein accumulation and replication of chloroplasts 10, Protein plastid movement impaired4.
FtsZ2 (cell visision protein FtsZ homolog 2) is required for plastid cell division. Present in two isoforms: FtsZ2-1 and FtsZ2-2. Alternative names: Plastid division protein FTSZ2.