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FtsZ1 and 2 | Plant cell division protein FtsZ1 and FtsZ2

AS09 413 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thalian, Hordeum vulgare

FtsZ1 and 2 | Plant cell division protein FtsZ1 and FtsZ2 in the group Antibodies for Plant/Algal  / Developmental Biology / Cytoskeleton at Agrisera AB (Antibodies for research) (AS09 413)

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Product Information

Immunogen

Recombinant part of Arabidopsis thalina FtsZ conserved in FtsZ1 Q42545 At5g55280 and FtsZ2 including FtsZ2-1 O82533, At2g36250 and FtsZ2-2 Q9LXJ0, At3g52750 and in a wide range of FtsZ proteins from other plant species.

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 2500 (WB)
Expected | apparent MW

40 kDa FtsZ1 (Arabidopsis thaliana) and 50 kDa FtsZ2 (Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare
Predicted reactivity Chlamydomons reinhardtii, Cucumis sativus, Gentiana lutea, Glycine max, Gossypium arobretum, Jatropha manihot, Lilium longiflorum, Lupinus angustifolius, Manihot esculenta, Marchantia aquatica, Medicago truncatula, Morus notabilis, Nannochloropsis gaditana, Nicotiana tabacum, Oryza sativa, Physcomitrella patens, populus trichocarpa, Ricinus communis, Solanum lycopersicum, Sorgum bicolor, Theobroma cacao, Triticum uRatum, Zea mays, Yellow gentian, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

western blot using plant anti-FtsZ1 and FtsZ2 antibody

2 µg
of total protein extracted with PEB (AS08 300) from leaf tissue of (1) Hordeum vulgare, (2) Arabidopsis thaliana, (3 - 5) recombinant FtsZ1/FtsZ2 (33 kDa) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-FtsZ1/FtsZ2 antibody (AS09 413, 1:2500, 1h) and secondary anti-rabbit (1:10 000, 1 h) antibody (HRP conjugated, secondary antibody AS09 602) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent, using a Fuji LAS-3000 CCD (300s, standard sensitivity). Exposure time was 2 min.

Additional information

Additional information This product can be sold containing proclin if requested.

Related products

Related products

AS07 217 | Anti-FtsZ | procaryotic cell division GTPase, cyanobacterial, rabbit antibodies
AS10 715 | Anti-FtsZ procaryotic cell division GTPase (bacterial), rabbit antibodies
AS13 2651 | Anti-FtsZ2 plant cell division protein ftsZ2, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Background

FtsZ1 (cell division protein FtsZ homolog 1) is required for plastid cell division. Localization: pollen grain and plastids of vegetative cells. Alternative names: Chloroplast FtsZ, Protein accumulation and replication of chloroplasts 10, Protein plastid movement impaired4.

FtsZ2 (cell visision protein FtsZ homolog 2) is required for plastid cell division. Present in two isoforms: FtsZ2-1 and FtsZ2-2. Alternative names: Plastid division protein FTSZ2.

Product citations

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